2001 Fiscal Year Final Research Report Summary
Molecular cloning of the Restorer gene for Cytoplasmic male-Sterility in Rice
| Project/Area Number |
12660008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Akita Prefectural University |
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Principal Investigator |
AKGI Hiromori Akita Prefectural University, Faculty of Bioresource Sciences, Associate Professor, 生物資源科学部, 助教授 (50315587)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Kouichi Akita Prefectural University, Faculty of Bioresource Sciences, Professor, 生物資源科学部, 教授 (70166354)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Rice / CMS / PPR protein / Rf gene / Hybrid / Microsatellite / Map-based cloning |
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| Research Abstract |
Cytoplasm male sterility and the fertility restoration system are indispensable to development of hybrid rice. In order to clarify the molecular mechanism of the CMS system, we decided to identify the restorer (Rf-1) gene for BT type CMS in rice by using a map-based cloning strategy. Linkage analysis with the DNA marker near the Rf-1 gene using the 6104 segregants delimited the Rf-1 gene locus to a 22.4-kb genomic region. In this region, we found out two ORF (Rf-1A and Rf-1B) encoding PPR proteins. We also cloned cDNA from each ORF. Sequence analysis revealed that several ins/del had occurred in both ORF of non-restoring genotype, which resulted in a production of nonfunctional protein. Rf-1A encodes a mitochondrially targeted protein almost entirely comprised 10 repeats of PPR motif. On the other hands, the Rf-1B gene encoded the protein lacked the signal peptide to a mitochondria. It is thought that PPR protein participates in processing of RNA of an organella and Rf-1 gene participate mRNA of the atp6 gene domain of a mitochondria in processing. It was concluded that one of the two ORF had been found in the Rf-1 locus, named as Rf-1A, encodes the Rf-1 protein which may be involved in the processing mRNA of the mitochondrial atp6 gene.
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