2001 Fiscal Year Final Research Report Summary
Active Oxygens Generation and the Formation of Scavenging System with Maturation and Senescence in Fruit and Vegetables
| Project/Area Number |
12660027
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | Yamaguchi University |
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Principal Investigator |
YAMAUCHI Naoki Faculth of Agriculture, Yamaguchi University, Professor, 農学部, 教授 (60166577)
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| Co-Investigator(Kenkyū-buntansha) |
IMAHORI Yoshihiro Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Associate Professor, 大学院・農学生命科学研究科, 講師 (40254437)
SHIGYO Masayoshi Faculty of Agriculture, Yamaguchi University, Associate Professor, 農学部, 助教授 (40314827)
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| Project Period (FY) |
2000 – 2001
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| Keywords | broccoli / sweet pepper / active oxygens / hydrogen peroxide / ascorbate-glutathione cycle / heat treatment / 成熟 / 老化 |
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| Research Abstract |
Active oxygens generation and the formation of scavenging system with maturation and senescence in sweet pepper and broccoli were studied.
1. The floret yellowing was inhibited in heat-treated (50℃, 2 hrs) broccoli. Hydrogen peroxide content in heat-treated broccoli showed higher level than the control. The activities of the enzymes involved in an ascorbate - glutathione cycle showed higher levels in heat-treated broccoli than those in control. Cytosolic ascorbate peroxidase (cAPX) activity in heat-treated broccoli increased slightly during storage at 15℃. Moreover, the cAPX mRNA level in heat-treated broccoli was detected greatly during storage.
2. Superoxide dismutase (SOD) activity in sweet pepper increased from green (G) to yellow/green (Y/G) stage of maturation. A number of Cu, Zn-SOD isozymes increased at green/yellow (G/Y) and Y/G stages. SOD activity was mainly found in cytosol. APX activity increased from G to Y/G stage. A number of cAPX isozymes increased from G/Y to Y/G stage and the activity was present chiefly in the cytosol. The enzyme activities involved in the ascorbate-glutathione cycle showed a change with maturation.
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