2001 Fiscal Year Final Research Report Summary
Functional Analysis of the Promoter for Phytoalexin Synthesis Gene
| Project/Area Number |
12660043
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | Nagoya University |
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Principal Investigator |
YOSHIOKA Hirofumi Nagoya University, Graduate School of Bioagricultural Science, Assistant Professor, 生命農学研究科, 助手 (30240245)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Phytoalexm / Sesquiterpene cyclase / Rishitm / Potato / Defensegene / Promoter |
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| Research Abstract |
Exposure of potato tuber tissues to incompatible race of Phytophthora infestans, a pathogen of potato late blight, causes multiple defense responses, including the oxidative burst, accumulation of sesquiterpenoid phytoalexins (lubimin and rishitin). All these reactions are also induced in potato tubers by the treatment with a crude elicitor preparation (hyphal wall component ; HWC) from the mycelium of the fungus. Sesquiterpene cyclase is a key enzyme in isoprenoid pathway for the synthesis of sesquiterpenoid phytoalexin. The expression of the gene in plant-pathogen interactions is not well documented. Here we isolated and characterized CDNA encoding potato sesquiterpene cyclase, vetispiradiene synthase (PVS). PVS was encoded by multiple gene family (PVS1-PVS4). As one molecular approach to elucidation of the mechanisms that regulate the phytoalexin synthesis in potato tubers, we investigated changes with time in the transcript levels of PVS. These members were stimulated by an incompatible and a compatible races of P. infestans. Interestingly, only PVS3 was induced in the potato leaves.
We isolated and characterized the PVS3 genes from a potato genomic library.Transient expression of luciferase (Luc) reporter gene under the control of PVS3 promoter showed that high level of Luc activity was induced by the fungal elicitor. These results indicated that the promoter region contains cis-acting element for defense response.
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[Publications] Yoshioka, H., Kuchimura, K. and Doke, N.: "China Agricultural Scientech Press, Beijing, China,"Molecular cloning, expression, and characterization of the cDNA and gene for sesquiterpene cyclase in potato., Shimai, Z., Guanghe, Z. and Huaifang, L.. 36-40 (2001)
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