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2001 Fiscal Year Final Research Report Summary

Mechanism of regulation of E. coli cell duplication by DNA-, RNA- binding protein Hfq

Research Project

Project/Area Number 12660069
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionTokyo Institute of Technology

Principal Investigator

WACHI Masaaki  Tokyo Inst. Technol., Dept. Bioengineering, Associate Professor, 大学院・生命理工学研究科, 助教授 (90192822)

Project Period (FY) 2000 – 2001
Keywordsiron ion / oxidative stress / Hfq / RNaseE / RNase G / mRNA
Research Abstract

FepA and FhuE proteins involved in iron transport in the outer-membrane were overproduced in the hfq::cat mutant cells. The hfq::cat mutant was more susceptible to killing by hydrogen peroxide, probably due to the excess incorporation of iron. Direct measurement of intracellular iron concentrations by electroparamagnetic resonance spectroscopy revealed that the mutant cells indeed accumulated significantly higher levels of iron than the wild type cells. The Hfq protein protected DNA from iron-induced oxidative damages in vitro. These results suggest that the Hfq protein is involved in the mechanism of cell survival by protecting cells from oxidative stress.
Genetic interaction between hfq and RNazes was examined. Temperature sensitivity of the ams-1 (RNaze E^<ts>) mutant strain was suppressed by introduction of the hfq::cat mutation. Its suppression was cancelled by introduction of mg::cat (RNaze G defective) mutation. These results suggest that the Hfq protein is involved in the mechanism of regulation of mRNA stability by RNaze E and RNaze G.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Fujishima, H.: "kdsA mutations affect FtsZ-ring formation in Fscherichia coli K-12"Microbiology. 148. 103-112 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wachi, M.: "A novel RNase G mutant that is defective in degradation of adhE mRNA but proficient in the processing of 16S rRNA"Biochem. Biophys. Res. Commun.. 289. 1301-1306 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wachi, M.: "Escherichia coli ribonuclease G"Methods Enzymol.. 342. 55-63 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirasawa, T.: "L-Glutamate production by lysozyme-sensitive Corynebacterium glutamicum ltsA mutant strains"BMC Biotechnol.. 1. 9 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Umitsuki, G.: "Involvement of RNase G in in vivo mRNA metabolism in Eschcrichia coli"Genes Cells. 6. 403-410 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 和地正明: "大腸菌RNaseG"日本農芸化学会誌. 75. 121-127 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wijayarathna, C.D.: "Isolation of ftsI and murE genes involved in peptidoglycan synthesis from Corynebactcrium glutamicum"Appl. Microbiol. Biotechnol.. 55. 466-470 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirasawa, T.: "A mutation in the Corynebacterium glutamicum ItsA gene causes susceptibility to lysozyme, temperature-sensitive growth, and L-glutamate production"J. Bacteriol.. 182. 2696-2701 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 和地 正明: "大腸菌の新規RNA分解酵素RNascGの発見とその機能解析"日本農芸化学会誌. 74 臨時増刊号. 445-446 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Fujishima, A. Nishimura, M. Wachi,k H. Takagi, T. Hirasawa, H. Teraoka, K. Nishimori, T. Kawabata, K. Nishikawa, and K. Nagai: "kdsA mutations affect FtsZ-ring formation in Escherichia coli K-12"icrobiology. 148. 103-112 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaaki Wachi, Naoko Kaga, Genryou Umitsuki, David P. Clark, and Kazuo Nagai: "A novel Rnase G mujtant that is defective in degradation of adhE mRNA but proficient in the processing of 16S rRNA"Biochem. biophys. Res. Commun.. 289. 1301-1306 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaaki Wachi, and Kazuo Nagai: "Escherichia coli ribonuclease G ; In A.W. Nicholson (ed.), Methods in Enzymology"Academic Press, Inc., New York. vol.342,Ribonucleases. 55-63 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takashi Hirasawa, Masaaki Wachi, and Kazuo Nagai: "L-glutamate production by lysozyme-sennsitive Corynebacterium glutamicum ltsA mutant strains"BMC Biotechnol. 1. 9 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Genryou Umitsuki, Masaaki Wachi, Ayako Takada, Takafusa Hikichi, and Kazuo Nagai: "Involvement of Rnase G in in vivo mRNA metabolism in Escherichia coli"Genes Cells. 6. 403-410 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Champika Dilrukshi Wijayarathna, Masaaki Wachi, and Kazuo Nagai: "Isolation of ftsI and murE genes involved in peptidoglycan synthesis from Corynebacterium glutamicum"Appl. Microbiol. Biotechnol.. 55. 466-470 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takashi Hirasawa, Masaaki Wachi, and Kazuo Nagai: "A mutation in the Corynebacterium glutanicum ltsA gene causes susceptibility to lysozyme, temperature-sensitive growth, and L-glutamate production"J. Bacteriol.. 182[10]. 2696-2701 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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