2002 Fiscal Year Final Research Report Summary
Morphological analysis of the cerebrospinal-fluid-(CSF)-contacting neuron in the lateral septum of avian species
| Project/Area Number |
12660270
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Basic veterinary science/Basic zootechnical science
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
HIRUNAGI Kanjun Nagoya University, Museum, Assoc.Prof., 博物館, 助教授 (00126898)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUKUTA Katuhiro Nagoya University, Graduate School of Agrical.Sciences, Prof., 大学院・生命農学研究科, 教授 (10012022)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Keywords | Japanese quail / Immunohistochemistry / Deep brain photoreceptor / VIP neuron / Suprachiasmatic nucleus / CSF-contcting neuron |
|---|
| Research Abstract |
We demonstrated clusters of immunoreactive small neurons in the lateral septum of the Japanese quail Opsin-like immunoreactive neurons and VIP-like immunoreactive neurons have similar morphological features. Their perikarya accumulate in the subependymal regions of the ventromedial walls of both lateral ventricles. The labelled neurons are multipolar or bipolar with pyriform or spindle-shaped cell bodies. Immunoreactive CSF-contacting neurons contact the CSF via a process that penetrates the ependyma and terminates in a single knob-like swelling. Scanning electron microscopic study revealed ventricular processes. Spherical bodies (about 2 μm in diameter) are found in a highly circumscribed region of the lateral ventricular wall of the medial lateral septum. These structures are distributed within about 1000 μm in rostro-caudal direction This structure is considerd as the venticular process of the CSF-contacting neuron. We examined immunoelectron microscopic analyses of neuronal elements in the suprachiasmtic nucleus (mSCN) of the Japanese quail using antibodies of vasoactive intestinal polypeptide (VIP), VIP receptor (VIPR) and an opsin. VIP ir elements are classified in two groups. About 40% of VIP ir neuronal elements are a synapse type. Another is a varicose type, which shows no synaptic specialization in the section. VIPR immunoreactivity is found as a fuzzy material on the membrane of the process, which seems to correspond to a synaptic junction. Opsin ir elements contain immunoreactive small granules. This neuronal element is probably a varicose of neuronal fiber. In several avian species, VIP and opsin co-exist in the deep brain photoreceptor. The presence of opsin ir terminals suggests that opsin ir deep brain photoreceptors of the lateral septum innervate the mSCN of the Japanese quail.
|
