2001 Fiscal Year Final Research Report Summary
Developmental toxicity of endocrine disruptors in the zebrafish embryo.
| Project/Area Number |
12660288
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | School of Veterinary Medicine, Rakuno Gakuen University |
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Principal Investigator |
HIRAGA Takeo School of Veterinary Medicine, Rakuno Gakuen University, Professor, 獣医学部, 教授 (30112577)
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| Co-Investigator(Kenkyū-buntansha) |
TERAOKA Hiroki School of Veterinary Medicine, Rakuno Gakuen University, Assistant Professor, 獣医学部, 講師 (50222146)
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| Project Period (FY) |
2000 – 2001
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| Keywords | endocrine disrupter / developmental toxicity / zebra fish / dioxin / AhR / apoptosis / hypoplasia of jaw / tributyl tin |
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| Research Abstract |
The effects of 2, 3, 7,8-tetxachbrodibenzovrdbxin (TCDD) and tributyl tin (TBT) exposure on central nervous system, lower jaw development and edema were investigated in early zebrafish (Danio rerio) embryos. The results are summarized as follow :
1.TCDD exposure induced the occurrence of apoptotic cell death during the early stage of development in the dorsal midbrain of zebrafish. Some experiments showed that TCDD induced apoptosis could be related to aryl hydrocarbon receptor (AHR) activation, induction of cytochrome P450 1A (CYP1A) and oxidative stress. Moreover, we determined the complete CDNA sequence of a CYP1A open reading frame with both 5'- and 3'-ends in zebrafish
2.TCDD retarded lower jaw development in a concentration-dependent manner. Initially, TCDD may act directly on the lower jaw development. Reductions in hypobranchial blood flow occurred after the initial retardation in lower jaw development had com apparent, and may contribute further to the effect. From the whole-mount in situ hybridization and immunohistochemical method, sonic hedgehog protein and its receptors are strongly suggested to be involved in a jaw development.
3.TBT exposure induced general edema caused by circulation failure in zebrafish embryos.
These results suggest that the zebrafish have many advantages as a useful vertebrate model to detect the toxicity of endocrine disrupters.
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Research Products
(18 results)
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[Publications] Dong, W., Teraoka, H., Kondo, S., Hiraga, T.: "2,3,7,8-tetrachlorodibenzo-p-dioxin induces apoptosis in the dorsal midbrain of zebrafish embryos by activation of aryihydrocarbon receptor"Neurosci. Lett.. 303. 169-172 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Teraoka, H., Maruyama, Y, Takehana, K., Iwanaga, T., Hiraga, T., Fujita, S., Ohta, T.: "Ca^<2+> signaling in porcine duodenal glands by muscarinic receptor activation"Am. J. Physiol.. 280. G729-G737 (2001)
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「研究成果報告書概要(和文)」より
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[Publications] Maruyama, Y., Teraoka, H., Iwata, H., Kazusaka, A., Fujita, S.: "Inhibitory effects of endogenous dopaminergic neurotoxin, norsalsolinol on dopamine secretion in PC12 rat pheochromocytoma cells"Neurochem. Int.. 38. 567-572 (2001)
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「研究成果報告書概要(和文)」より
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[Publications] Teraoka, H., Dong, W., Ogawa, S., Tsukiyama, S., Okuhara, Y., Niiyama, M., Ueno, N., Peterson, R.E., Hiraga, T.: "2,3,7,8 -Tetrachlorodibenzo-p-dioxin toxicity in zebrafish embryo : Altered regional blood flow and impaired lower jaw development"Toxicol. Sci.. 65. 192-199 (2002)
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「研究成果報告書概要(和文)」より
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[Publications] Yamazaki, K., Teraoka, H., Dong, W., Stegeman, J.J., Hiraga, T.: "cDNA cloning and expression of cytochrome P450 1A in zebrafish embryos"J. Vet. Med. Sci., Accepted with revision.
Description
「研究成果報告書概要(和文)」より
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[Publications] Dong, W., Teraoka, H., Yamazaki, K., Tsukiyama, S., Imani, S., Imagawa, T., Stegeman, J.J., Peterson, R.E., Hiraga, T.: "2,3,7,8-Tetrachlorodibenzo-p-dioxin toxicity in the zebrafish embryo : Local circulation failure in the dorsal midbrain is associated with increased apoptosis"Toxicol. Sci., Accepted with revision.
Description
「研究成果報告書概要(和文)」より
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[Publications] Teraoka H., Dong W., Ogawa S., Tsukiyama S., Okuhara Y., Niiyama M., Ueno N., Peterson RE. & Hiraga T: "2, 3, 7, 8-Tetrachbrodibenzo-/rdioxin toxicity in zebrafish embryo : Altered regional blood flow and impaired lower jaw development"Toxicol. Sci.. 65. 192-199 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Dong W., Teraoka H., Yamazaki K., Tsukiyama S., Imani S., Imagawa T., Stegeman J. J., Peterson R E. & Hiraga T.: "2, 3, 7, 8 - Tetrachlorodibenzo-p-dioxin toxicity in the zebrafish embryo : Local circulation failure in the dorsal midbrain is associated with increased apoptosis"Toxicol. Sci.. (Accepted with revision.). (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Dong W., Teraoka H., Tsukiyama S., Yamazaki K., Imani S., Imawawa T., Kondo S. & Hiraga T.: "2, 3, 7, 8, - tetrachlorodibenzo-P-dioxin induces apoptosis in central nervous system: Correlation between local circulation failure and apoptosis in the dorsal midbrain of zebrafish embryos"J. Rakuno Gakuen Univ.. (in press). (2002)
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「研究成果報告書概要(欧文)」より
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