2001 Fiscal Year Final Research Report Summary
Histochemical study on the specific antibody for lymphatic endothelial cells and endothelial growth factors
| Project/Area Number |
12670021
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | OITA MEDICAL UNIVERSITY |
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Principal Investigator |
KATO Seiji Oita Medical University, Department of Anatomy, Professor, 医学部, 教授 (60034956)
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| Co-Investigator(Kenkyū-buntansha) |
CHENG Ji Rui Oita Medical University, Department of Anatomy, Assistant Professor, 医学部, 助手 (60305034)
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| Project Period (FY) |
2000 – 2001
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| Keywords | lymphatics / 5'-nucleotidase / microcirculation / histochemistry / blood vessel / endothelial growth factor / monoclonal antibody / diaminopeptidase |
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| Research Abstract |
The microcirculatory system, lymphatics and blood vessels was histochemically studied in regards to the organ specificity of the structural organization and fine distribution of the lymphatic capillary network. The lymphatics and blood vessels are characterized by an enzyme-histochemical method using 5'-nucleotidase (5'-Nase), alkaline phosphatase (ALPase) and / or diaminopeptidase (DAPase) staining in addition to an immunohistochemical method. The 5'-Nase-positive lymphatic vessels could be distinguished histochemically from arterial and venous vessels based on ALPase and DAPase activity, respectively. The specificity and localization of enzyme reactions were confirmed by comparative histochemical studies of the same specimen under light microscope and scanning or transmission electron microscope. Furthermore, this report presents a new monoclonal antibody for 5'-Nase which we developed in our laboratory. These enzyme-and immunohistochemical methods are discussed in relation to their ability to demonstrate the organ specificity of developing vascular networks under normal and pathological condition.
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