2001 Fiscal Year Final Research Report Summary
THE ROLE IF THE MOUSE mSOX7 GENE IN CARDIAC MUSCLE CELLS
| Project/Area Number |
12670030
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Keio University |
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Principal Investigator |
HIRAOKA Yoshiki Keio University, School of Medicine, Lecturer, 医学部, 専任講師 (80218768)
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| Project Period (FY) |
2000 – 2001
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| Keywords | CARDIAC MUSCLE CELL / EMBRYOGENESIS / TRANSCRIPTION FACTOR / SOX GNE |
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| Research Abstract |
SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcriptswas estimatedtobe 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary. The antibody also reacted to cardiac muscle cells.
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