2001 Fiscal Year Final Research Report Summary

Functional analyses of ATF-2 gene family members by using knockout-mouse

Research Project

Project/Area Number	12670126
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	General medical chemistry
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	MAEKAWA Toshio RIKEN, Molecular Genetics Laboratory, Senior Scientist, 分子遺伝学研究室, 副主任研究員 (90201764)
Project Period (FY)	2000 – 2001
Keywords	Transcription factor / ATF-2 / Mammary tumour / GADD45 / APC / c-jun / Anisomycin / Knockoutmouse
Research Abstract	We have generated and anaiyzed the mutant mouse lacking transcription factor ATF-2 gene far members. 1.ATF-2 heterozygously knockout female mouse developed mammary tumours at very high ratio 18/ : Most of these tumours were adenocarcinoma scirrhus. 2, By using Tip-Technology, we have found that the expressions of APC and c-jun are reduced sev folds in ATF-2 deficient MEF. 3, We have found that the induction of GADD45 gene by anisomycin is inhibited in the ATF-2 defic cell line. 4, The expression levels of GADD45 gene were much reduced in the tumours than in normal mammal 5, ATF-2 homozygously and heterozygously knockout MEFs that express activated RAS develo tumours in nude-mouse at 100%, but wild type MEF that express activated RAS couldn't develop tumours. 6, From these results we can say that ATF-2 heterozygously knockout femaie mouse have high potent to develop tumours.

(4 results)