2001 Fiscal Year Final Research Report Summary
Structure and function of a new member of ectophosphodiesterase I
| Project/Area Number |
12670135
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kobe University |
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Principal Investigator |
SANO Kimihiko KOBE UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, Lecturer, 大学院・医学系研究科, 講師 (40205993)
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| Project Period (FY) |
2000 – 2001
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| Keywords | CD203c / cancer metastasis / cancer infiltration / E-NPP1 / E-NPP3 / bile duct carcinoma ; cholangiocarcinoma ; carcinogenesis |
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| Research Abstract |
E-NPP (ecto-nucleotide pyrophosphatase/phosphodiesterase I) consists of three closely related molecules. E-NPP1 (PC-1) was first cloned as a plasma cell differentiating antigen. E-NPP-2 (autotaxin) was purified as an autocrine tumor cell motility stimulation factor from melanoma cells. And E-NPP3 was cloned from human prostate CDNA library. Forced overexpression of E-NPP3 gives fibroblasts and glioma cells an invasive property. We have investigated the expression and localization of E-NPP1 and 3 in human neoplastic bile duct diseases. Western blot analysis revealed that expression of E-NPP3 but not E-NPP1 was higher in tumor tissues than in surrounding tissues. Immunohistochemical analysis demonstrated that E-NPP1 was located at apical side of tumor cells while E-NPP3 was at the apical plasma membrane. Cleaved form of E-NPP3 protein but not E-NPP1 was readily detected in the cholangiocarcinoma patients' sera. In situ hybridization confirmed the presence of E-NPP1 and -3 mRNA in tumor tissues. Furthermore, NIH3T3 cells that stably transfected with E-NPP3 CDNA (NIH3T3/E-NPP3 cells) but not NIH3T3/E-NPP1 cells showed increased migration rate through collagen-coated membranes. These results suggest that E-NPP3 is involved in tumor cell infiltration of bile duct carcinoma and cholangiocarcinoma.
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Research Products
(21 results)
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[Publications] Johnson,k K.A., Hessle, L., Vaingankar, S., Wennberg, C., Maruo, S., Narisawa, S., Goding, J.W., Sano, K., Millan, J.L., and Terkeltunb, R: "Osteoblast tissue-nonspecific alkaline phosphatase (TNAP) antagonizes and regulates PC-1"Am.J. Physiol. 279(10). R1365-1377 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hasegawa, D., Sano, K., Kosaka, Y., Hayakawa, A., Kawagoe, R., Kawasaki, K., and Nakamura, H.: "Acute megakaryoblastic leukemia in an infant with a novel translocation, t(1;9)(p32;q34)"Cancer Genet. Cytogenet. 122(1). 59-62 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] KUmaki, S., Ishii, N., Minegishi, M., Ohashi, Y., Hakozaki, I., Nonoyama, S., Imai, K., Morio, T., Tsuge, I. Sakiyama, Y., Miyanoshita, A., Miura, J., Mayumi, M., Heike, T., Katamura, K., Takada, H., Izumi, I., Kamizono, J., Hibi, S., Sasaki, H., Kimura, M., Kikuta, A., Date, Y., Sako., M., Tanaka, H., Sano, K., Sugamura, K.: "Characterizationof the c chain among 27 unrelated Japanese patients with X-linked severe combined immunodeficiency (X-SCID)"Hum Genet.. 107. 406-408 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Rutsch, F., Vaingankar, S., Johnson, K.A., Goldfine, I., Maddux, B., Schauerte, P., Kalhoff, H., Sano, K., Boisvert, W.A., Superti-Furga, A., and Terkeitaub, R.: "PC-1 Nucleoside triphosphate pyrophosphohydrolase deficiency in idiopathic infantile arterial calcification"Am. J. Pathol.. 158(2). 543-554 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Hayakawa, A., Matsuda, Y., Daibata, M., Nakamura, H., and Sano, K.: "Genomic organization, tissue expression, and cellular localization ofr AF3p21, a fusion partner of MLL in therapy-related leukemia"Gene Chromosome Cancer. 30(4). 364-374 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Buhring, H.-J., Seiffert, M., Giesert, C., Marxer, A., Valent, P., Kanz, L., and Sano, K.: "The basophil activation marker defined by antibody 97A6 is identical with the ecto-nucleotide pyrophosphatase 3(E-NPP3 ; CD203c)"Blood. 97(10). 3303-3305 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] sugahara, K., Iyama, K.-I., Kimura, T., Sano, IK., Darlington, G.J., Akiba, T., and Takiguchi, M.: "Mice lacking CCAAT/enhancer-binding protein a show hyperproliferation of alveolar type II cells and increased sufactant protein mRNAs"Cell Tissue Res.. 306. 57-63 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Hara, J., Park, Y.-D., Yoshioka, A., Yumura-Yagi, K. Koudera, U., Hosoi, G., Sako, M., Kosaka, Y., Sano, K., Hideo, M., Mabuchi, O.., Aoyagi, N., Yamamoto, M.,m Tawa, A., Miyata, H., Tanaka, H., Kikkawa, M., Shimodera, M., and Kawa-Ha, K.: "Intensification of chemotherapy using block therapies as consokidation and reinduction therapies for acute lymphoblastic leukemia during childhood"Int. J. Hematol. 74. 165-172 (2001)
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「研究成果報告書概要(欧文)」より
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