Expression of NF-Y in cancer tissue and its relation to cell proliferation

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 12670185
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Okinaka Memorial Institute for Medical Research (2002); University of Occupational and Environmental Health, Japan (2000-2001)
• Principal Investigator: TANIMOTO Akihide Okinaka Memorial Insitut for Medical Research, researcher, 研究員 (10217151)
• Co-Investigator(Kenkyū-buntansha): SASAGURI Yasuyuki Univ, Occup, Environ. Health, Dept. Pathol., professor, 医学部, 教授 (60140646)
• Project Period (FY): 2000 – 2002
• Keywords: cancer / NF-Y / Cell proliferation

Research Abstract

The expression of NF-Y protein was detected in the cancer tissues of the breast, prostate, and stomach using a catalyzed signal amplification system for immunohistochemistry. Especially, gastric cancer cells showed a higher ratio of NF-Y expression in their nuclei, and also those of normal gastric epithelial in proliferative phase. The immunolocalization of proliferating nuclear antigen and MIB-1 were very consist with that of NF-Y. This suggested that the NF-Y is a potential new maker for gastric cancer cell proliferative activity. The cdc2 expression, which is regulated by NF-Y, was not detected in the cancer cells, possibly due to the antibody specificity we used for immunostain.
The cdc2 gene promoter activity in cultured human gastric cancer cells was reduced by dominant negative NF-Y.
Taken together, we showed that the NF-Y regulates the cdc2 gene promoter activity and that its expression is high in gastric cancer cell.