2001 Fiscal Year Final Research Report Summary
Pathogenic role of heat shock protein of Helicobacter pylori and host immune response to the heat shock protein
| Project/Area Number |
12670265
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Kyorin University |
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Principal Investigator |
KAMIYA Shigeru Dept. Infect. Dis., Kyorin Univ. Sch. Med. Professor, 医学部, 教授 (10177587)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Hiroyuki Dept. Infect. Dis., Kyorin Univ. Sch. Med. Lecturer, 医学部, 講師 (40221650)
TAGUCHI Haruhiko Dept. Infect. Dis., Kyorin Univ. Sch. Med. Lecturer, 医学部, 講師 (20146541)
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| Project Period (FY) |
2000 – 2001
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| Keywords | H. pylori / heat shock protein / cytokine / vaccine / epitope |
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| Research Abstract |
By using purified heat shock protein 60 (HSP60) of Helicobacter pylori and fusion protein of HSP60 with beta-galactosidase, it was shown that H. pylori HSP60 stimulated a secretion of interleukin-8 (IL-8) which is closely related with the induction of gastric mucosal inflammation from gastric epithelial cells. In addition, purified HSP60 induced apoptosis of gastric epithelial cells. These results indicate that HSP60 of H. pylori might play an important role in pathogenesis following H. pylori infection.
An epitope reactable with monoclonal antibody (Mab) to HSP60 was detected on the surface of gastric epithelial cells. Immune response to H. pylori HSP60 was stronger in the sera of the patients with H. pylori infection than in those without H. pylori infection. Not only fusion protein of HSP60 with beta-galactosidase but also oligopeptide pH9 reactable with the Mab showed a protective effect on colonization of H. pylori in mice. These results suggest that HSP60 of H. pylori might be a good candidate as a component of vaccine
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