2001 Fiscal Year Final Research Report Summary
TCR gene rearrangement in the peripheral lymphoid organs
| Project/Area Number |
12670293
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | CHIBA UNIVERSITY |
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Principal Investigator |
NAKAYAMA Toshinori Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (50237468)
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| Project Period (FY) |
2000 – 2001
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| Keywords | T cell receotor / rearrangement / Peyer's Patch / RAG2 / GFP-knock-in |
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| Research Abstract |
We have established GFP (green fluorescence protein) gene knock-in Recombination activating gene-2 (RAG-2) -knockout animals to detect TCRαβgene rearrangement in the peripheral lymphoid organs. RAG-2 is essential for gene rearrangement of both antigen receptors of T and B cells. To identify T cell subpopulations undergoing TCR rearrangement in the peripheral lymphoid organs other than the thymus, we established mice in which a GFP gene is knocked in the RAG-2 gene locus (RAG2-GFP mice). In the thymus and bone marrow of RAG2-GFP mice, as expected, GFP expression was detected in appropriate stages of developing T cells and B cells. Interestingly, a fraction of Thyl.2^+ cells in the Peyer's Patch are found to be GFP-positive. The GFP-positive cells express high levels of surface TCRβ and CD3 on the surface, suggesting mature T cells with rearranged TCRαβ. However, significant levels of signal-end breaks of Jα and Dβ locus were detected by ligation-mediated PCR analysis. Moreover, circular DNAs generated from TCRα and TCRβ gene locus were detected at levels comparable to those of total thymocytes. These results suggest that there exist secondary rearrangement events of TCR α and β gene in a fraction of the Peyer's Patch T cells.
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Research Products
(12 results)
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[Publications] Kaneko, Y., Harada, M., Kawano, T., Yamashita, M., Shibata, Y, Gejyo, F., Nakayama, T., Taniguchi, M.: "Augmentation of Va14 NKT cell-mediated cytotoxicity by IL-4 in an autocrine mechanism resulting in the development of concanavalin A-induced hepatitis."J. Exp. Med.. 191. 105-114 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamashita, M., Katsumata, M., Iwashima, M., Kimura, M., Shimizu, C., Kamata, T., Shin, T., Seki, N., Suzuki, S., Taniguchi, M., Nakayama, T.: "TCP-induced calcineurin activation regulates Th2 cell development by modifying the IL-4 receptor signaling complex."J. Exp. Med.. 191. 1869-1879 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Matsuda, J. L., Naidenko, O. V., Gapin, L., Nakayama, T., Taniguchi, M., Wang, C-R., Koezuka, Y., Kronenberg, M.: "Tracking the response of NKT cells to a glycolipid antigen using CD1d tetramers : The response to a galactosylceramide is dynamic and highly compartmentalized."J. Exp. Med.. 192. 741-754 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Shimoda, K., Kato, K., Aoki, K., Matsuda, T., Miyamoto, A., Shibamori, M., Yamashita, M., Numata, A., Takase, K., Kobayashi, S., Shibata, S., Asano, Y, Gondo, H., Sekiguchi, K., Nakayama, K., Nakayama, T., Okamura. T., Okamura, S., Niho, Y., Nakayama, K.: "Tyk2 plays a restricted role in IFNa signaling although it is required for IL-12-mediated T cell function."Immunity. 13. 561-571 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kimura, M., Koseki, Y., Yamashita, M., Watanabe, N., Shimizu, C., Katsumoto, T, Kitamura, T, Taniguchi M., Koseki, H., Nakayama, T.: "Regulation of Th2 cell differentiation by mel-18, a mammalian polycomb group gene."Immunity. 15. 275-287 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Sharif, S., Arreaza, G. A., Zucker, P., Mi, Q.-S., Sondhi, J., Naidenko, O. V., Kronenberg, M., Koezuka, Y., Delovitch, T. L., Gombert, J.-M., Leite-de-Moraes, M., Gouarin, C., Zhu, R., Hameg, A., Nakayama, T., Taniguchi, M., Lepault, F., Lehuen, A., Bach, J.-F., Herbelin, A.: "Activation of natural killer T cells by a galactosylceramide treatment prevents the onset and recurrence of autoimmune Type 1 diabetes."Nature Medicine. 7. 1057-1062 (2001)
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「研究成果報告書概要(欧文)」より
