Epidemological study on the relationship between ischemia heart disease and anti-CagA antibody of Helicobacter pylori.

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 12670334
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Fukuoka University
• Principal Investigator: MIYAZAKI Motonobu School of Medicine, Fukuoka University Assistant Professor, 医学部, 助教授 (60299542)
• Co-Investigator(Kenkyū-buntansha): MOMOSE Yoshito School of Medicine, Fukuoka University Research Assistant, 医学部, 助手 (20123119) ; UESHIBA Hajime School of Medicine, Fukuoka University Instructor, 医学部, 講師 (60232740) ; UNE Hiroshi School of Medicine, Fukuoka University Professor, 医学部, 教授 (40122676) ; IWAHASHI Mitsue School of Medicine, Fukuoka University Research Assistant, 医学部, 助手 (70330909)
• Project Period (FY): 2000 – 2001
• Keywords: Helicobacter pylori / CagA / Ischemic heart disease

Research Abstract

The purpose of this case-control study, carried out by examining for the presence of anti-CagA antibodies, was to investigate the relationship between ischemic heart disease (IHD) and Helicobacterpylori (H. pylori) infection. Enrolled subjects were 242 patients 94 controls. All cases were angiographically confirmed stenoses of coronary artery. Of 242 patients, patients with acute coronary syndrome (ACS) were 164. A control case is with the result of 'within normal limit' on coronary angiography. H. pylori seropositivity was determined with an IgG-specific enzyme II assay kit (Biomerica, Newport Beach, CA). Antibodies to CagA were determined with an enzyme-linked immunosorbent assay (ELISA). The antigen was a recombinant CagA antigen, which was supplied by Dr. Antonello Covacci (Immunobiological Research Institute, Siena, Italy). The logistic regression model was applied to analyze a relationship between an ACS and a control in regard to H. pylori seropositivity. Odds ratios (ORs) and their 95% confidence intervals (95% CIs) were determined to evaluate the strength of the association. Probability values less than 0.05 were considered significant. The data were analysed with the Statistical Analysis System (SAS Institute, Gary, NC). The seropositivity for H. pylori in ACS group was 70.4% and it in controls was 72.1%. No statistically significant difference was observed (OR= 0.92, 95% CI ; 0.51-1.67, p= 0.073). Of 100 H. pylori-seropositive ACS cases, 64.0% was CagA positive and in 62 H. pylori-serbpositive controls, 66.1% was CagA positive. There was not statistically significant difference about the anti-CagA antibodies (OR= 0.91, 95% Cl ; 0.47-1.77, p= 0.783).