Expression of Th2-specific chemokines in bronchial asthma

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 12670415
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 内科学一般
• Research Institution: The University of Tokyo
• Principal Investigator: ISHII Akira The University of Tokyo, Graduate School of Medicine, Lecturer, 医学部・附属病院, 講師 (30272553)
• Co-Investigator(Kenkyū-buntansha): HIRAI Koichi The University of Tokyo, Graduate School of Medicine, Visiting Associate Professor, 大学院・医学系研究科, 客員助教授 (10156630)
• Project Period (FY): 2000 – 2001
• Keywords: bronchial asthma / allergy / Th2 cells / chemokine / TARC / CCL17 / MDC / CCL22 / CCR4 / bronchial epithelial cells

Research Abstract

We investigated the expression of Th2-specific chemolines, i. e., TARC (thymus and activation regulated chemokine) / CCL 17 and MDC (macrophage-derived chemokine) / CCL22 in bronchial asthma. Bronchial epithelium of asthmatics but not normal exhibited intense immunoreactivity with TARC. MDC immunoreactivty was only weak in both asthmatic and normal epithelium, and no difference of staining intensity was observed in both groups. Bronchial epithelial cell line cells liberated ng/ml quantities of TARC but not MDC in response to combined stimulation with IL-4/TNF-α. (Sekiya. T, et al. J. Immunol.65 : 2205-13, 2000)
TARC levels in sputum and serum in asthmatics was increased compared to those in normals. On the other hand, no significant difference was observed in MDC level in both group. (Seliya. T, et al. Allergy, in press)

Research Products

• [Publications] Sekiya, T., M.Miyamasu, A.Ishii, K.Hirai., et al.: "Inducible expression of a Th2-type CC Chemokine thymus and activation regulated chemokine (TARC) by human bronchial epithelial Cells"J. Immunol.. 165. 2205-2213 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ohta, K., N.Yamashita, A.ishii, T.Miyamoto., et al.: "In vivo effects of apoptosis in asthma examined by a murine model"Int. Archi. Allergy Immunol.. 124(1-3). 259-261 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sekiya, T., H.Yamada, A.Ishii, K.Hirai., et al.: "Increased levels of a Th2-type CC Chemokines thymus and activation-regulated chemokine (TARC) in duced sputum and serum of asthmatics"Allergy. 57(in press). (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshimura, C., M.Yamaguchi, A.Ishii, K.Hirai., et al.: "Activation markers of human basophils : CD69 expression is strongly and preferentially induced of by IL-3"J. Allergy Clin. Immunol.. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sekiya T, M. Miyamasu, M. Imanishi, H. Yamada, T. Nakajima, M. Yamaguchi, T. Fujisawa, R. Pawankar, Y. Sano, K. Ohta, A. Ishii, Y. Morita, K. Yamamoto, K. Matsushima, O. Yoshie, and K. Hirai: "Inducible expression of a Th2-type CC chemokine thimus-and activation-regulated chemokine (TARC) by human bronchial epithelial cells"J. Immunol. 165. 2205-2213 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ohta K, N. Yamashita, M. Tajima, T. Miyasaka, R. Kawashima, J. Nakano, H. Arioka, A. Ishii, T. Horiuchi, and T. Miyamoto: "In vivo effects of apoptosis in asthma examined by a murine model"Int. Archi. Allergy Immunol. 124 (1-3). 259-61 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sekiya T, H. Yamada, A. Morita, M. yamaguchi, A. Ishii, K. Matsushima, O. Yoshie, Y. Sano, K. Yamamoto, and K. Hirai: "Increased levels of a Th2-type CC Chemokines thymus and activation-regulated chemokine (TARC) in induced sputum and serum of asthmatics"Allergy. 57 (in press). (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yoshimura C, M. Yamaguchi, M. Iikura, S. Izumi, K. Kudo, H. Nagase, A. Ishii, T. Iwata, T. Igarashi, K. Yamamoto, and K. Hirai: "Activation markers of human basophils : CD69 expression is strongly and preferentially induced of by IL-e"J. Allergy Clin. Immunol. (in press). (2002)
  • Description: 「研究成果報告書概要(欧文)」より