2001 Fiscal Year Final Research Report Summary
Expression of heparanase in the hepatic tissue of hepatitis patients and its clinical significance
| Project/Area Number |
12670509
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
ITOH Yoshito Kyoto Prefectural University of Medicine, Research Associate, 医学部, 助手 (70244613)
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| Project Period (FY) |
2000 – 2001
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| Keywords | chemokine receptor / heparanase / hepatitis / hepatoma cell lines |
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| Research Abstract |
In chronic hepatitis C (CH-C) patients, the ratio of CXC chemokine receptor 3 (CXCR3) positive peripheral blood mononuclear cells (PBMC) increased during the exacerbation of the hepatitis. In some interferon (IFN)-alpha treated CH-C, the ratio of CXCR3 positive PBMC also increased during the IFN treatment. The expression of heparanase in the hepatic tissues as well as in the PBMC is under investigation.
The expression of chemokine receptors and heparanase (CCR1〜10、CXCR1〜4) in 6 cell lines derived from human hepatoma(HuH-7, PLC/PRF/5, HepG2, Hep3B, HLE, HLF) and Chang liver cell were examined by Taq Man PCR. The mRNA expression of 4 chemokine receptors (CCR6, CCR11, CXCR4, CXCR11) increased in several of the hepatoma cell lines, and the mRNA expression of heparanase increased in all hepatoma cell lines except for HepG2.
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