2000 Fiscal Year Annual Research Report

脳内の内因性神経ペプチドが肝・胆・肺の生理機能および病態に及ぼす作用に関する研究

Research Project

Project/Area Number	12670512
Research Institution	Dokkyo Medical University
Principal Investigator	米田政志獨協医科大学, 医学部, 助手 (30261407)
Keywords	肝病態 / 神経ペプチド / 神経伝達物質 / 肝障害
Research Abstract	急性肝障害時の脳内の神経ペプチドの関与を検討した。Wistar系雄性ラット(220-280g)を24時間の絶食の後、CRF antagonistであるα-helical CRF(0.125,0.25,0.5,1,2,5μg)および生理食塩水をそれぞれCCl4(2.0ml/kg sc)投与直前と6時間後に脳槽内投与した。24時間後にCO2にて屠殺し、血清ALT,AST,ALP,T-Bil値を測定し、肝を取り出してH-E染色に組織学的検討も加えた。その結果、生理食塩水の脳槽内投与は肝障害に影響を及ぼさなかったが、α-helical CRFの脳槽内投与によりCCl4による血清ALT値の上昇は用量依存性に抑制された(Mean±SE;IU/L:生理食塩水330±21;α-helical CRF0.125μg342±13;0.25μg246±22;0.5μg223±19;1μg181±20;2μg137±15;5μg139±3;n=5-9)。同様にα-helical CRF(2μg)の投与によりCCl4による血清AST,ALP,T-Bil値の上昇も抑制され、更に壊死や脂肪変性といった組織変化も軽減した。6-ヒドロキシドーパミン(100mg/Kg,ip7日前と80mg/Kg,ip4日前)および肝交感神経切除(3日前)により中枢性α-helical CRFによる本肝障害防御作用は消失したが、アトロピン(0.15mg/kg,ip)および迷走神経肝臓枝切断術(3日前)は影響しなかった。

Research Products

(8 results)

All Other

All Publications (8 results)

[Publications] Hizume T,Watanobe H,Yoneda M,Suda T,Schioth H: "Involvement of Prolactin-releasing peptide in the preovulatory luteinizing hormone and prolactin surges in the rat"Biochem Biophys Res Commun. 279(1). 35-39 (2000)
[Publications] Kamiya K,Kono T,Iwamoto J,Yoneda M,Kotani H,Kasai S: "The cytoprotective role of lipopolysaccharide-induced nitric oxide against liver damage during early phase of endotoxemia in rats"Shock. 14(2). 229-233 (2000)
[Publications] Kono T,Nishida M,Inagaki N,Tanaka Y,Yoneda M and Kasai: "Development and Characterization of 1C6-203, a New Monoclonal Antibody Specific to Human Thymidine Phosphorylase"J Histochem Cytochem. 49(1). 131-137 (2001)
[Publications] Watanobe H,Yoneda M,Kakizaki Y,Kohsaka A,: "Further evidence for a significant participation of the melanocortin 4 receptor in the preovulatory prolactin surge in the rat"Brain Res Bull. (印刷中).
[Publications] Yoneda M and Terano A: "Central regulation of hepatic function by neuropeptides"Journal of Gastroenterology. (印刷中).
[Publications] Watanobe H,Yoneda M: "A signufucant participation of leukemia inhibitory factor in regulating the reproductive function in rats : a novel action of the pleitropic cytokine"Biochem Biophys Res Comm. (印刷中).
[Publications] 米田政志,黒澤美枝子,寺野彰: "Annual Review消化器2000"中外医学社. 378 (2000)
[Publications] 米田政志,寺野彰: "Annual Review消化器2001"中外医学社. 340 (2001)

2000 Fiscal Year Annual Research Report

脳内の内因性神経ペプチドが肝・胆・肺の生理機能および病態に及ぼす作用に関する研究

Principal Investigator

米田 政志 獨協医科大学, 医学部, 助手 (30261407)

Research Products

[Publications] Hizume T,Watanobe H,Yoneda M,Suda T,Schioth H: "Involvement of Prolactin-releasing peptide in the preovulatory luteinizing hormone and prolactin surges in the rat"Biochem Biophys Res Commun. 279(1). 35-39 (2000)

[Publications] Kamiya K,Kono T,Iwamoto J,Yoneda M,Kotani H,Kasai S: "The cytoprotective role of lipopolysaccharide-induced nitric oxide against liver damage during early phase of endotoxemia in rats"Shock. 14(2). 229-233 (2000)

[Publications] Kono T,Nishida M,Inagaki N,Tanaka Y,Yoneda M and Kasai: "Development and Characterization of 1C6-203, a New Monoclonal Antibody Specific to Human Thymidine Phosphorylase"J Histochem Cytochem. 49(1). 131-137 (2001)

[Publications] Watanobe H,Yoneda M,Kakizaki Y,Kohsaka A,: "Further evidence for a significant participation of the melanocortin 4 receptor in the preovulatory prolactin surge in the rat"Brain Res Bull. (印刷中).

[Publications] Yoneda M and Terano A: "Central regulation of hepatic function by neuropeptides"Journal of Gastroenterology. (印刷中).

[Publications] Watanobe H,Yoneda M: "A signufucant participation of leukemia inhibitory factor in regulating the reproductive function in rats : a novel action of the pleitropic cytokine"Biochem Biophys Res Comm. (印刷中).

[Publications] 米田政志,黒澤美枝子,寺野彰: "Annual Review消化器2000"中外医学社. 378 (2000)

[Publications] 米田政志,寺野彰: "Annual Review消化器2001"中外医学社. 340 (2001)

米田政志獨協医科大学, 医学部, 助手 (30261407)