2001 Fiscal Year Final Research Report Summary
analysis of cytoprotection by COX-2 induced in gastric mucosa : influence by selective COX-2 inhibitors
| Project/Area Number |
12670526
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
MIYAKE Kazumasa Nippon Medical School, Third department of Internal Medicine, Assistant, 医学部, 助手 (60247012)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAMOTO Choitsu Nippon Medical School, Third department of Internal Medicine, Assistant, 医学部, 教授 (30196092)
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| Project Period (FY) |
2000 – 2001
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| Keywords | cyclooxygenase-2 / selective COX-2 inhibitors / gastric mucosal injury / ethanol / NS 398 / Indomethacin |
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| Research Abstract |
Purpose : Endogenous and exogenous prostaglandins (PGs) have been shown to contribute to reduce gastric injury caused by irritants given subsequently. The aim of the study is to clarify whether cyclooxygenase-2 (COX-2) protein induced by pretreatment is involved in prevention of subsequent ethanol-caused gastric injury in mice. Methods : Mice were pretreated with acidified ethanol or saline and then COX-2 protein expression was immunohistochemically determined at every 8 hours in the stomachs. Mice were administered 95 % ethanol 24 hours after acidified ethanol pretreatment and gastric mucosal damages were evaluated macroscopically and histologically. The effects of NS-398 or indomethacin on 95 % ethanol-caused damage were also examined. Results : Acidified ethanol pretreatment induced COX-2 protein expression in lamina propria macrophages of the gastric mucosa with its peak level 24 hours after the pretreatment. 95 % ethanol caused gastric mucosal damages. The degree of the damages was not different between mice pretreated with acidified ethanol and saline. However, NS-398 aggravated ethanol-caused damages only in mice pretreated with acidified ethanol, while indomethacin aggravated the damages evaluated histologically Irrespective of the pretreatment. Conclusions : The pretreatment- induced COX-2 in addition to COX-1 seems to be involved in the defense mechanism through minimizing damages caused by a subsequent irritant.
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Research Products
(13 results)
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[Publications] Miyake K, Tsukui T, Wada K, Tatsuguchi A, Futagami S, Hiratsuka T, Shinoki K, Iizumi T, Akamatsu T, Sakamoto C, Kobayashi M.: "Irritant-induced cyclooxygenase-2 is involved in the defence mechanism of the gastric mucosa in mice"J Gastroenterol. 37(3) (in press). (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Miyake K, Tsukui T, Futagami S, Tatsuguchi A, Shinoki K, Hiratsuka T, Iizumi T, Nagata K, Shinji Y, Wada K, Yamada N, Kobayashi M, Sakamoto C.: "Effect of acid suppression therapy on development of gastric erosions after cure of Helicobacter pylori infection"Aliment Pharmacol Ther. 16 (Suppl.2). 1-7 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tsukui T, Kashiwagi R, Sakane M, Tabata F, Akamatsu T, Wada K, Futagami S, Miyake K, Sueoka N, Hirakawa T, Kobayashi M, Fujimori T, Sakamoto C.: "Aging increases and duodenal ulcer reduces the risk for intestinal metaplasia of the gastric corpus in Japanese patients with dyspepsia"Journal of Gastroenterology and Hepatolopy. 16. 15-21 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Tatsuguchi A, Sakamoto C, Wada K, Akamatsu T, Tsukui T, Miyake K, Futagami S, Kishida T, Fukuda Y, Yamanaka N, Kobayashi M.: "Localization of cyclooxygenase-1 and cyclooxygenase-2 in Helicobacter pylori-related gastritis and gastric ulcer tissues in humans"Gut. 46(6). 782-789 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Tatsuguchi A, Sakamoto C, Fukuda Y, Wada K, Akamatsu T, Tsukui T, Miyake K, Futagami S, Kishida T, Yamanaka N, Kobayashi M.: "Induction of cyclooxygenase-2 in mesothelial cells in peritonitis caused by perforated ulcers - an immunohistochemical study in humans"Aliment Pharmacol Ther. 14 (Suppl. 1). 58-63 (2000)
Description
「研究成果報告書概要(欧文)」より
