2002 Fiscal Year Final Research Report Summary
PAHTOGENESIS ON MITOCHONDRIAL ANGIOPATHY AND ITS REPAIR BY GENE INJECTION
| Project/Area Number |
12670598
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Neurology
|
|---|
| Research Institution | FUKUI MEDICAL UNIVERSITY |
|---|
Principal Investigator |
YONEDA Makoto FUKUI MED. UNIV., University Hospital, LECTURER, 医学部附属病院, 講師 (70270551)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KURIYAMA Masaru FUKUI MED. UNIV., Faculty of Medicine, PROFESSOR, 医学部, 教授 (80107870)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Keywords | MELAS / ANGIOPATHY / CYBRIDS |
|---|
| Research Abstract |
Mitochondrial encephalplathy, myopathy, lactic acidosis and strokelike episodes (MELAS) syndrome is characterized by a mitochondrial DNA (mtDNA) mutation (A3243G) and mitochondrial angiopathy in the brain. To investigate the pathogenic mechanism for mitochondrial angiopathy, we tried to construct cybrids that carry the MELAS mutation and were derived from human brain vessel endothelial cells. First, we determined the optimal concentration of ethidium bromide (EtBr) to reduce or remove mtDNA from the blood vessel endothelial cells. We then introduced the platelet mitochondria from MELAS patients into the blood vessel endothelial cells lacking mtDNA. Cybrids carrying the mtDNA mutation were hard to survive. We should further investigate the optimal media condition to get cybrids survived.
|
-
-
-
-
-
-
[Publications] Tanaka, M, Borgeld, HJ, Zhang, J, Muramatsu, S, Gong, JS, Yoneda M, Maruyama, W, Naoi, M, Ibi, T, Sahashi, K, Shamoto, M, Fuku, N, Kurata, M, Yamada, Y, Nishizawa, K, Akao, Y, Ohishi, N, Miyabayashi, S, Umemoto, H, Muramatsu, T, Furukawa, K, Kikuchi, A, Nakano, I, Ozawa, K, Yagi, K: "Gene Therapy for Mitochondrial Disease by Delivering Restriction Endonuclease Smal into Mitochondria"J Biomed Sci. 9. 534-541 (2002)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Yoneda, M, Fujita, T, Yamada, Y, Yamada, K, Fujii, A, Inagaki, T, Nakagawa, H, Shimada, A, Kishikawa, M, Nagaya, M, Azuma, T, Kuriyama, M, Wakamatsu, N: "Late infantile Hirschsprung disease-mental retardation syndrome with a 3-bp deletion in ZFHX1B"Neurology. 59. 1637-1640 (2002)
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Yoshida, K, Yamazaki, H, Ozeki, T, Inoue, T, Yoshioka, Y, Yoneda, M, Fujiwara, Y, Inoue, T: "Mitochondrial genotypes and radiation-induced micronucleus formation in human osteosarcoma cell in vitro"Oncology Report. 53. 615-619 (2001)
Description
「研究成果報告書概要(欧文)」より
-
