2002 Fiscal Year Final Research Report Summary
Energy source in brain activation monitored by nuclear magnetic resonance
| Project/Area Number |
12670623
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
IGARASHI Hironaka Nippon Medical School, School of Medicine, Assistant Professor, 医学部, 講師 (20231128)
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| Project Period (FY) |
2000 – 2002
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| Keywords | MRI / MRS / rCBF / CMRO2 / rCBV / CMRglu / brain metabolism / NMR |
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| Research Abstract |
Goal of this study is to quantify the activation of glycolysis and oxygen consumption in activated brain region, simultaneously using magnetic resonance (MR). We developed following MR methods, and applied to measure the metabolic state in normal and pathologic brain.
1. CMRglu measurement using 1H[13C] magnetic resonance spectroscopy
First we developed high special resolution magnetic resonance spectroscopic imaging with which we succeeded to quantify the brain metabolites from 0.94x0.94x4mm region in vivo. Appling this methods, we measured the CMRglu in normal rat brain (0.31±0.08γmol/min/g) which is almost identical to the value obtained with other methods.
2. Oxygen consumption (CMRO2) measurement
【1!○】 17O tracer study
We developed indirect H2[17]O concentration mapping using spin lock forst imaging in 4sec time resolution and 0.45 x 0.9 mm in spatial resolution. To assess the feasibility of this methods we measure the regional cerebral blood flow (rCBF) using H2[17]O as a tracer. rCBF of cortex using this method was 175ml/100g/min.
【2!○】 BOLD theory delivered CMRO2 measurement
We assessed alternative method, which does not need the external tracer. Basically this method is based on the blood oxygen level dependent (BOLD) method. Using this technique, we measure the changes of oxygen extraction ratio (OEF) in normal and ischemic brain. Since CMRO2 is delivered multiplying OEF with rCBF. We also assessed the rCBF measurement using spin of blood flow as a tracer (continuous spin labellin (CASL) method). Using these two methods together changes of regional CMRO2 could be mapped.
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Research Products
(19 results)
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[Publications] Igarashi H, Hamamoto M, Yamaguchi H, Ookubo S, Nagashima J, Nagayama H, Amemiya S, Arii K, Sakamaki M, Katayama Y: "Diffusion - Perfusion mismatch during the acute-stage of cerebral ischemia may predict enlargement of the infarcted area"J. Cerebral Blood Flow and Metabolism. 19, Supple 1. 352 (2001)
Description
「研究成果報告書概要(欧文)」より
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