2001 Fiscal Year Final Research Report Summary
RESEARCH OF THE STRUCTURE AND BINDING BETWEEN T CELL RECEPTOR AND ANTIGEN-PRESENTING MOLECULE IN THE ALLERGIC DISEASE
| Project/Area Number |
12670742
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | GIFU UNIVERSITY |
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Principal Investigator |
INOUE Ryosuke GIFU UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF PEDIATRICS, ASSISTANT PROFESSOR, 医学部・附属病院, 助手 (60273132)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Zenichiro GIFU UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF PEDIATRICS, ASSISTANT PROFESSOR, 医学部, 助手 (90303502)
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| Project Period (FY) |
2000 – 2001
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| Keywords | BETA-LACTOGLOBULIN / ANTIGEN-SPECIFIC T CELL / HLA / TCR / CDR3 / IL-4 / IFN-GAMMA RATIO / ANALOGUE-PEPTIDE THERAPY |
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| Research Abstract |
(1) Six TCCs established from the five patients responded to three different peptide fragments, and the fragment of BLGp97-117 was recognized most frequently by the T cells. BLGp101-112 (KYLLFCMENSAE) was the core sequence to be recognized in this portion.
(2) Milk allergy group was higher than control group in possession ratio of HLA-DRB1^*0405 allele, but there was not significant difference between both groups. HLA-DRB1^*0405 allele participate in BLG-recognition profoundly.
(3) Sequence analysis of TCR with RT-PCR method revealed a striking heterogeneity and similar patterns of amino acid sequence were recognized in the CDR3 region.
(4) Analysis of cytokine production revealed high levels of IL-5 production by antigen-specific T cell lines of all ; IL-4 and IFN-γ were also produced in a variety of degree by these T cell lines. The differences in the ratios of IL-4 to IFN-γ might explain the differences in their clinical features.
(5) We substituted another amino acid for an amino acid of BLGp101-111 by one and composed several analogue peptides. The T cell clone reacted to only the peptides with similar property. Biochemical property of the antigen can prescribe the T cell response in the allergic patho-genesis. We aim development of the novel therapy using analogue peptides that induce Th1-typed T-cell response.
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Research Products
(19 results)
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[Publications] Fukao T., Yoshida T., Kaneko H., X-Q., Song., Tashita, H., Teramoto T., Inoue R., Watters D., Lavin M., Kondo N.: "Immunoblot analysis for laboratory diagnosis of ataxia-telangiectasia : Use of Epstein-Barr virus-transformed or phytohemagglutinin-stimulated lymphoblasts for detection of ATM protein"J. Invest. Allergol. Clin. Immunol.. 10. 36-40 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kondo N., Matui E., Kaneko H., Kato Z., Fukao T., Teramoto T.,Inoue R., Watanabe M., Sakaguchi H., Kasahara K., Morinoto N.: "Role of IL-12 receptor β 2 mutations in the development of atopy"ACI International supplement. No. 1. 92-94 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Fujii H., Fukutomi O., Inoue R., Shinoda S., Okamoto H., Teramoto T., Kondo N., Wada H., Saito K., Matsuoka T., Seishima M.: "Autonomic regulation after exercise evidenced by spectral analysis of heart variability in asthmatic children"Ann Allergy Athma Immunol.. 85. 233-237 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kondo N., Matui E., Kaneko H., Kato Z., Fukao T., Teramoto T., Inoue R., Watanabe M., Sakaguchi H., Kasahara K., Morimoto N.: "Reduced interferon-γ production and mutations of the IL-12 receptor β 2 chain gene in atopic subjects"Int. Arch. Allergy. Immunol.. 124. 117-120 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Watanabe M., Kaneko H., Shikano H., Aoki M., Sakaguchi H., Matui E., Inoue R., Kato Z., Kasahara K., Fukutomi O., Kondo T., Kondo N.: "Predominant expression of 950delCAG of IL-18R alpha chain cDNA is associated with reduced IFN-gamma production and high serum IgE level in atopic patients"J. Allergy Clin. Immunol.. (in press.). (2002)
Description
「研究成果報告書概要(欧文)」より
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