2001 Fiscal Year Final Research Report Summary
Cell Cycle Dependence of Boron Uptake in Boron Compounds Used for Clinical Neutron Capture Therapy
| Project/Area Number |
12670852
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | UNIVERSITY OF TSUKUBA |
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Principal Investigator |
YOSHIDA Fumiyo College of Medical Technology and Nursing, University of Tsukuba, Research Associate, 医療技術短期大学部, 助手 (30261811)
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| Co-Investigator(Kenkyū-buntansha) |
TAKANO Shingo Department of Immunology, Institute of Clinical Medicine, University of Tsukuba, Assistant Professor, 臨床医学系, 講師 (50292553)
MATSUMURA Akira Department of Immunology, Institute of Clinical Medicine, University of Tsukuba, Assistant Professor, 臨床医学系, 講師 (90241819)
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| Project Period (FY) |
2000 – 2001
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| Keywords | cell cycle / flow cytometry / sodium borocaptate / boronophenylalanine / neutron capture therapy |
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| Research Abstract |
In neutron capture therapy, it is important to incorporate boron in tumor cells selectively. The present study used flow cytometry to sort the cells by G0/G1 phase and G2/M phase, and the boron concentration of each fraction was measured with ICP-AES. The results revealed that BSH and BPA, had higher rates of boron uptake in the G2/M than in the G0/G1. However, the difference was more prominent with BPA. The G2/M : G0/G1 ratio decreased as a function of time in culture in BPA, thereby indicating the cell cycle dependency of BPA uptake. Such heterogeneity of boron uptake in tumor cells should be considered for microdosimetry.
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