2001 Fiscal Year Final Research Report Summary
Research and Development of Apoptosis Detection by PET.
| Project/Area Number |
12670853
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Gunma University |
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Principal Investigator |
INOUE Tomio Gunma University, Associate Professor, 医学部, 助教授 (80134295)
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| Co-Investigator(Kenkyū-buntansha) |
ENDO Keigo Gunma University School of Medicine, Professor, 医学部, 教授 (10115800)
ORIUCHI Noboru Gunma University School of Medicine Assistant Professor, 医学部, 講師 (40292586)
AOKI Jun Gunma University School of Medicine Associate Professor, 医学部, 助教授 (80212364)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Apoptosis / PET / Annexin |
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| Research Abstract |
Apoptotic death is a phenomenon in cellular unit, and the development of imaging the apoptotic death would be useful for resolving unknown pathophisiology of various disease, diagnoses, and therapeutic decision. The nuclear medicine imaging using radio nuclides might be the most sensitive and possible technology for imaging molecular biologic phenomenon such as apoptosis. In these technique, positron emission tomography (PET) has been focused because of its high quantitative imaging technology. This study was conducted in the PET facility in Gunma University. The aim of this study was to basically and clinically evaluate the relationship between the F-18 α methyltyrosine (FMT) as a amino-acid transporter marker, C-11 choline as a cellular membrane phospholipid metabolic marker and apoptotic phenomenon. Also we tried to develop new C-11 or F-18 labeled PET tracers detecting apoptotic phenomenon on in vivo images which were belonged to a phospholipids family. As a result, we could not find a significant correlation between FMT and C-11 choline tumor uptake and apoptotic phenomenon from clinical data analysis. We succeeded to generate a new tracer. C-11 Annexin, detecting apoptotic phenomenon. This new tracer showed a significant different cell biding ability between apoptosis free cells and apoptosis induced cells.
C-11 Annexin appeared to be an arailable for detecting apoptotic cells and further investigation using in vivo animal study was warranted before clinical trials.
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