2001 Fiscal Year Final Research Report Summary
GENOMIC DISCORDANCE BETWEEN MONOZYGOTIC TWINS DISCORDANT FOR AUTISM
| Project/Area Number |
12670945
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | DIVISION OF NEUROPSYCHIATRY, DEPARTMENT OF TRANSLATIONAL MEDICAL SCIENCES NAGASAKI UNIVERSITY GRADUATE SCHOOL OF BIOMEDICAL SCIENCES |
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Principal Investigator |
TSUJITA Takahiro DIVISION OF NEUROPSYCHIATRY, DEPARTMENT OF TRANSLATIONAL MEDICAL SCIENCES NAGASAKI UNIVERSITY GRADUATE SCHOOL OF BIOMEDICAL SCIENCES, ASSISTANT PROFESSOR, 医学部, 講師 (40304919)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANE Yoshibumi DIVISION OF NEUROPSYCHIATRY, DEPARTMENT OF TRANSLATIONAL MEDICAL SCIENCES NAGASAKI UNIVERSITY GRADUATE SCHOOL OF BIOMEDICAL SCIENCES, PROFESSOR, 医学部, 教授 (80039833)
YOSHIURA Koichiro DEPARTMENT OF HUMAN GENETICS, NAGASAKI UNIVERSITY SCHOOL OF MEDICINE, LECTURER, 医学部, 助手 (00304931)
NIIKAWA Norio DEPARTMENT OF HUMAN GENETICS, NAGASAKI UNIVERSITY SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (00111170)
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| Project Period (FY) |
2000 – 2001
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| Keywords | AUTISM / MONOZYGOTIC TWINS / METHYLATION / EPIGENETICS / RLGS (Restriction Landmark Genome Scanning) / FRGP (Fluorescent Representational Genomic Profiling) |
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| Research Abstract |
Genomic DNA of monozygotic twins discordant for autism was analyzed to determine whether their genomes were truly identical. The subjects were monozygotic female twins, one of whom had DSM-IV Autistic disorder. Genomic DNA was extracted from leukocytes and was applied to FRGP (Fluorescent Representational Genomic Profiling) analysis. After comparisons of patterns with approximately 600 spots, we detected at least four spots with intensities that obviously differed in the two twins. The discrepancies likely were generated by differences in the methylation status of DNA between the twins. The difference may influence the transcription level of one or more genes.
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