2001 Fiscal Year Final Research Report Summary

Molecular biological study on the organic solvent dependence -its mechanism and treatment-

Research Project

Project/Area Number	12670953
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Psychiatric science
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	FUKUI Kenji Kyoto Prefectural University of Medicine, Psychiatry, Professor, 医学部, 教授 (50165263)
Project Period (FY)	2000 – 2001
Keywords	organic solvent / psychiatric dependence / mesolimbic DA system / CREB / p-CREB / molecularbiology
Research Abstract	Since psychiatric dependence induced by the abuse of organic solvent is deeply related with the dopamine transmission, the mechanism of phospholylation of cAMP response element (CRE)-binding protein (CREB) was studied using the anti-CREB and anti-phospholylated CREB immunocytochemistry. In toluene-treated rat, the phospholylated-CREB (p-CREB) immunoreactive density increased in the nuclei in the nerve cell. The p-CREB immunoreactive cells were found in the entorinal cortex, prefrontal cortex, cingulated cortex, temporal cortex and hippocampus. In addition, the numbers of p-CREB immunoreactive cell have a tendency to the increment but the significant change is not obtained at present. The p-CREB-immunoreactive regions were closely distributed in the area of mesolimbic dopamine pathway, it was suggested that the phospholylation of CREB might have a close relation with the mechanism of organic solvent psychiatrid dependence.

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