2002 Fiscal Year Final Research Report Summary
Analysis of novel chemokine "WHCHE" in development and hematopoies
| Project/Area Number |
12670997
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | University of Tsukuba (2001-2002)
Kumamoto University (2000) |
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Principal Investigator |
OHNEDA Osamu University of Tsukuba, Institute of Clinical Medicine, Assistant Professor, 基礎医学系, 助教授 (30311872)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Chemokine / Endothelial cell / Flk-1 / WHCHE(CXCL15) / Transgenic mouse |
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| Research Abstract |
A novel chemokine 'WECHE' (CXCLI 5) was isolated from a dorsal aorta-derived endothelial stromal cell line. It was reported previously that WECHE inhibits the proliferation of yolk sac-derived endothelial cells and the differentiation of fetal liver-derived hematopoletic cells. In order to investigate a role of WECHE in vivo, WECHE was overexpressed in vivo by using Adenovirus system and transgenic mouse.
(1) Adenovirus system
Adenovirus was constructed by the ligation of WECHE cDNA under the controlled of chicken beta-actin promoter. The Adeno-WECHE was infected to E13.5 embryos and fetal liver was analyzed at E17. WECHE infected fetal liver contains two-fold higher number of CFU-E compared to the control (Adeno-LacZ).
(2) Transgenit mouse
CAAG 'promoter was used to generate WECHE transgenic mouse. Over fifty numbers of mice were analyzed, and it was found that one mouse has the transgene by PCR analysis. Then, several kinds of organs were extracted for mRNA, and WECHE expression was analyzed by RT-PCR. None of WECHE mRNA was observed in the transgenic mouse. We are going to generate Flk-1-WECHE transgenic mouse in order to examine the effect of VVECHE for endothelial cell development in vivo.
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Research Products
(14 results)
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[Publications] Ohneda O, Ohneda K, Nomlyama H, Zheng Z, Gold SA, Arai F, Miyamoto T, Taillon BE, Mclndoe RA, Shimkets RA, Lewin DA, Suda T, Lasky LA.: "WECHE: a novel hematopoletic regulatory factor."Immunity. 12. 141-150 (2000)
Description
「研究成果報告書概要(欧文)」より
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