2001 Fiscal Year Final Research Report Summary
BIOCHEMICAL MARKERS SPECIFIC FOR FETAL DISTERSS AND ITS PATHOGENETIC SIGNIFICANCE
| Project/Area Number |
12671071
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | NATIONAL INSTITUTE OF Neuroscience, NATIONAL CENTER OF NEUROLOGY AND PSYCHIATRY(NCNP) |
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Principal Investigator |
SAKURAGAWA Norio NATIONAL INSTITUTE OF Neuroscience, NCNP, DEPARTMENT OF METABOLIC DISEASES DIRECTOR, 神経研究所・疾病研究第五部, 部長 (70183374)
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| Project Period (FY) |
2000 – 2001
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| Keywords | FERAL DISTRESS / AMNIOTIC FLUID / CATECHOLAMINES / NEUROTROPHIC FACTORS / ACTIVIN |
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| Research Abstract |
TO INVESTIGATE THE BIOCHEMICAL MARKERS OF FETAL DISTRESS, WE EXAMINED NEUROTROPHIC FACRTORS IN AMNIOTIC FLUID FROM RATS RECEIVED TRANSIENT UTERIN ISCHEMIA. WE USED PREGNANCY 18 WISTER RATS, WHICH RIGHT UTERINE ARTERIS CRAMPED 30 MINUTES. AMNIOTIC FLUID OBTAINED BY AMNIOCENTESIS AFTER 24 HRS FROM CRAMPED. FOR EACH EXPERIMENT THE AMNIOTIC FLUID IN THE RIGHT HORN SERVED AS A ISCHEMIA GROUP AND THOSE IN THE LEFT HORN WERE USED AS A CONTROL GROUP. MEAHWHILE, WE PERFORMED IN VITRO EXPERIMENT FOR HYPOXIA. WE USED HUMAN AMNIOTC EPITHELAL CELLS DERIVED FROM PLACENTA BY CAESAREAN SECTION. CONTROL GROUP CULTURED IN 5% C02 INCUBATOR AT 37^<0>C FOR 48 HRS, ISCHEMIA GROUP INCUBATED IN 2% O_<2>, 5% CO_<2> INCUBATOR 37^<0> : FOR 48 HRS. STATISTICAL ANAYSIS BY WILCOXON SIGNED-RANK TEST DEMONSTRATED A SIGNIFICANT INCREASE IN NT-3 CONCENTRATIONS IN AMNIOTIC FLUID FROM THE ISHCEMIC GROUP IN CONTRAST NGF AND BDNF CONCENTRATIONS WHICH ALSO INCREASED BUT NOT SIGNIFICANT. IN VITRO EXPERIMNT SHOWED THAT NT-3 IN CULTURED MEDIUM OF HYPOXIC CELLS INCREASED SIGNIFICATLY. THESE RESULTS INDICATE THAT NT-3 IN AMNIOTIC FLUID MAY BE USED AS ONE OF BIOCHEMICAL MARKERS FOR FETAL DISTERSS.
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[Publications] Kosuga M, Okuyama T, Takahashi S, Sasaki K, Enosawa S, Li X-K, Okuyama S, Fujino M, Suzuki S, Yamada M, Matsuo N, Sakuragaw N: "Pnenotype correction in murine mucopolysaccharidosis type VII by transplantation of human amniotic epithelial cells after adenovirus-mediated gene transfer."Cell Transplantation. 9. 687-692 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kosuga M, Sasaki K, Li X-K, Ohkawa H, Ogino I, Okuda O, Arai H, Sakuragaw N, Kamata Y, Azuma N, Suzuki S, Yamada M, Okuyama T: "Engrafmemt of genetically-engineered amniotic epithelial cells corrects lysosomal storage multiple areas of the brain in mucopolysaccharidosis type VII mice."Mol Therapy. 3. 139-148 (2001)
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[Publications] Kosuga M, Takahashi S, Tanabe A, Fujino M, Li X-K, Suzuki S, Yamada M, Kakishita K, Ono F, Sakuragaw N, Okuyama T.: "Widespread distribution of adenovirus-transduced monkey amniotic epithelial cells after local intra-cerebral injection : Implication for cell-mediated therapy for lysosome storage disorders."Cell Transplant. 10. 435-439 (2001)
Description
「研究成果報告書概要(欧文)」より
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