2002 Fiscal Year Final Research Report Summary

Analysis of thyroid function in rats specifically expressing MHC class II and B7.1 in the thyroid

Research Project

Project/Area Number	12671076
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Endocrinology
Research Institution	University of Yamanashi Faculty of Medicine
Principal Investigator	ENDO Toyoshi University of Yamanashi, Faculty of Medicine, Assistant Professor, 医学部, 講師 (00152017)
Co-Investigator(Kenkyū-buntansha)	SHIMURA Hiroki University of Yamanashi, Faculty of Medicine, Research Associate, 医学部, 助手 (40303416)
Project Period (FY)	2000 – 2002
Keywords	Graves' disease / MHC class II antigen / B7.1 / transgenic rat / thyroid stimulating antibody / T3
Research Abstract	In order to investigate the role of MHC class II antigen and co-stimulatory molecule, B7.1, in the pathogenesis of Graves' disease, we prepared thyroglobulin promoter derived class II transactivator gene (CIITA) and also B7.1 gene. We produced transgenic rats of these gene. No difference was observed in the serum T3 between CIITA transgenic and the control rats, and we could not observed thyroid stimulating antibody (TSAb) activity in the sera of these transgenic animals. We further obtained transgenic rats specifically expressing both MHC class II and B7.1 in the thyroid. However, no TSAb activity was also observed in these rats. These results indicate that co expression of thyrotropin receptor and MHC class II antigen in thyroid gland is not sufficient to produce TSAb.

(4 results)