2002 Fiscal Year Final Research Report Summary
Analysis of thyroid function in rats specifically expressing MHC class II and B7.1 in the thyroid
| Project/Area Number |
12671076
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | University of Yamanashi Faculty of Medicine |
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Principal Investigator |
ENDO Toyoshi University of Yamanashi, Faculty of Medicine, Assistant Professor, 医学部, 講師 (00152017)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMURA Hiroki University of Yamanashi, Faculty of Medicine, Research Associate, 医学部, 助手 (40303416)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Graves' disease / MHC class II antigen / B7.1 / transgenic rat / thyroid stimulating antibody / T3 |
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| Research Abstract |
In order to investigate the role of MHC class II antigen and co-stimulatory molecule, B7.1, in the pathogenesis of Graves' disease, we prepared thyroglobulin promoter derived class II transactivator gene (CIITA) and also B7.1 gene. We produced transgenic rats of these gene. No difference was observed in the serum T3 between CIITA transgenic and the control rats, and we could not observed thyroid stimulating antibody (TSAb) activity in the sera of these transgenic animals. We further obtained transgenic rats specifically expressing both MHC class II and B7.1 in the thyroid. However, no TSAb activity was also observed in these rats. These results indicate that co expression of thyrotropin receptor and MHC class II antigen in thyroid gland is not sufficient to produce TSAb.
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