2001 Fiscal Year Final Research Report Summary
Molecular mechanisms of the disruption of immunological tolerance in type 1 diabetes
| Project/Area Number |
12671083
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | HYOGO COLLEGE OF MEDICINE |
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Principal Investigator |
NAMBA Mitsuyoshi Hyogo College of Medicine, Assoc. Prof., 医学部, 助教授 (00183533)
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| Co-Investigator(Kenkyū-buntansha) |
KONYA Hiroyuki Hyogo College of Medicine, Research Associate, 医学部, 助手 (50340956)
HAMAGUCHI Tomoya Hyogo College of Medicine, Research Associate, 医学部, 助手 (60330461)
KOHRU Kousuke Hyogo College of Medicine, Research Associate, 医学部, 助手 (30278832)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Insulitis / Pancreatic β-cell / Pancreatic biopsy / Betacellulin / Alloxan Dibetes |
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| Research Abstract |
(1) To explore the efficacy of regenerative approach against the pancreatic /3 -cell in the treatment of human type 1 diabetes, we examined the in vivo effect of recombinant human Betacellulin(BTC) on glucose intolerance and pancreatc morphlogy using a new mouse model with glucose intolerance induced by selective alloxan per fusion. Glucose tolerance was significantly improved and islet-like cell clusters, consisting mainly of $-cells, were increased in the pancreas by 4-weeks subcutaneous injections of ETC.
(2) To better understand the pathogenesis of type 1diabetes, we have developed pancreatic biopsy under laparoscope for recent -onset type 1 diabetic patients. T-cell-predominant infiltration to islets(insulitis) and hyperexpression of major histocompatibility complex clas 1 antigens on islet cells were observed without serious complications. Thus, the pancreatic biopsy under laparoscopy is a safe procedure for detecting in situ autoimmune phenomena in resent-onset type 1 diabeticpatients."
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