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2002 Fiscal Year Final Research Report Summary

Platelet-microparticles as a carrier of chemical mediators inducing remote organ failure

Research Project

Project/Area Number 12671155
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionOsaka University

Principal Investigator

KAWASAKI tomio  Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (90214626)

Co-Investigator(Kenkyū-buntansha) HIROSHI ogura  Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (70301265)
Project Period (FY) 2000 – 2002
Keywordsmicroparticles / PMN / sepsis / theology / deep vein thrombosis / pulmonary embolism / APCR
Research Abstract

The chemical mediators are secreted from activated platelets. The mediators actually made a play on peripheral vessels and sometimes made remote organ failure even though those materials are instantaneously resolved in blood. We have identified that the microparticles (MP) released from activated platelets were a transporter of the chemical mediators. In order to clarify the release mechanism and physiological meaning of MP the following projects were performed. MP production, platelet P selectin expression, platelet-monocyte binding, and platelet-polymorphonuclear cell binding increased in number in systemic inflammatory syndrome. Anti-P-selectin antibody clearly inhibited platelets-polymorphonuclear cell aggregation. In addition, leukocyte-platelet interaction and endothelial-MP binding were increased in patients with septic condition. Moreover, when the biorheology of the whole blood from septic patients was analyzed with the microarray bloodstream analyzer, the stiffness of white blood cells was increased. The result indicated that migration of white blood cells through the vessel wall was thought to be impaired. The white blood cells, stiffened by inflammation, may be trapped in the local stasis lesion. The platelet and MP bonded to the white blood cells made a new bonding to the other white blood cells. As a result, quite a new mechanism was advocated that start of coagulation accumulates the chemical mediators in inflammatory lesion. Moreover, it was clarified that the cause of activated protein C resistance (APC-R) in venous thrombosis in our country was not due to factor V Leiden gene mutation as was reported in Europe and America. It is not antibodies to phospholipids but anti-protein S antibodies that express APC-R in Japan. And this is the first report in the world (British Journal of Haematology 2002, 118, 577-583,Thromb Haemost 2002, 88, 716-722).

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Suehisa F, Toku M., Kawasaki T., Kanakura Y.: "Measurement of a newly developed thrombomodulin addition activated partial thromboplastin time assay in patients with deep venous thrombosis"Haemostasis. 31. 26-31 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nojima J., Kuratsune H, Suehisa E, Kawasaki T., Machii T., Kitani T., Iwatani Y., Kanakura Y.: "Acquired activated protein C resistance is associated with the co-existence of anti-prothrombin antibodies and lupus anticougulant activity in patients with systemic lupus erythematosis"British J. Hematology. 118. 557-583 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nojima J., Kuratsune H, Suehisa E, Kawasaki T., Machii T., Kitani T., Iwatani Y., Kanakura Y.: "Acquired activated protein C resistance associated with anti-protein S antibody as a strong risk factor for DVT in non-SLE patients"Thromb. Haemost.. 88. 716-722 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogasawara, N., Kijima, Y., Ike, S., Nakagawa, Y., Takagi, T., Hata, T., Suehisa, E., Kawasaki, T., Miyata, T.: "Hereditary protein S deficiency with a history of recurrent myocardial infarction. -A case report-"Circ. J.. 67・2. 166-168 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogasawara, N., Kijima, Y., Ike, S., Nagagawa, Y., Takagi, T., Hata, T., Suehisa, E., Kawasaki, T., Miyata, T.: "Hereditary protein S deficiency with a history of recurrent myocaridial infarction. -A case report-"Orc. J.. 67(2). 166-168 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nojima J., Kuratsune H., Suehisa E., Kawasaki T., Machii T., Kitani T., Iwatani Y., Kanakura Y.: "Acquired activated protein C resistance is associated with the co-existence of anti-prothrombin antibodies and hapus anticougulant activity in patients with systemic hupus erythematosus."British J. Haematology. 118. 577-583 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nojima J., Kuratsune H., Suehisa R., Kawasaki T., Machii T., Kitani T., Iwatani Y., Kanakuna Y.: "Acquired activated protein C resistance associated with anti-protein S antibody as a strong risk factor for DVT in non-SLE patients."Thromb. Haemost. 88. 716-722 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogura, H., Kawasaki, T., Tanaka, H., Koh, T., Tanaka, R., Ozeki,Y., Hosotsubo, H., Kuwagata, Y., Shimazu, T., Sugimoto, H.: "Activated platelets enhance miaoparticle formation and platelet-leukocyte interaction in severe trauma and sepsis."J. Trauma. 50(5). 801-809 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suehisa, E., Nomura, T., Kawasaki, T., Kanakura, Y.: "frequency of natural coagulation inhibitor (antithrombin III, Protein C and protein S) deficiencies in Japanese patients with spontaneous deep vein thrombosis."Blood Coagul Fibrinolysis. 12. 95-99 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suehisa E., Toku M., Kawasaki T., Kanakura Y.: "Measurement of a newly developed thrombomodulin addition activated partial thromboplastin time assay in patients with deep venous thrombosis."Haemostasis. 31. 26-31 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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