The Role of Tissue Factor (TF) In the development of Ischemic-reperfusion injury in Organ Transplantation

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 12671168
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: YOSHIMURA Norio Kyoto Prefectural University of Medicine Dept of Organ Transplantation and Endocrine Surgery Professor, 医学部, 教授 (00191643)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Masahiko Kyoto Prefectural University of Medicine Dept of Organ Transplantation and Endcrine Surgery Associate Professor, 医学部, 講師 (90295650)
• Project Period (FY): 2000 – 2002
• Keywords: Tissue Factor / ischemic-reperfusion injury / Organ Transplantation / アンチセンス法

Research Abstract

(Background.) Tissue factor (TF) is an initiation factor for blood coagulation, and its expression is induced on endothelial cells during inflammatory or immune responses. We designed an antisense oligodeoxynucleotide (AS-1/TF) for rat TF, and studied its effect on hepatic ischemic reperfusion injury
(Methods.) AS-1/TF was delivered intravenously to Lewis rats. After 10 hours, hepatic artery and portal vein were partially clamped. Livers were reperfused after 180 minutes and were harvested. TF expression was studied using immunohistochemical staining
(Result.) One of 10 rats survived in a 5-day survival rates and TF was strongly stained on endothelial cells in nontrearment group. However, by treatment with AS-1/TF, six of seven survived and TF staining was significantly reduced. Furthermore, we observed that fluoroscein labeled AS-1/TF was absorbed into endothelial cells.
(Conclusions.) These results suggest that AS-1/TF can strongly suppress the expression of TF and thereby inhibit ischemic reperfusion injury to the rat liver

Research Products

• [Publications] Matsuyama M, Akioka K, Okamoto M, Kadotani Y, Ushigome H, Nakamura K, Yoshimura R, Ohmori Y, Yoshimura N: "The Role of Tissue Factor In Renal Ischemic Reperfusion Injury by Antisense Oligodeoxynucleotide"Transplantation proceeding. 34. 1475-1476 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ushigome H, Sano H, Okamoto M, Kadotani Y, Nakamura K, Akioka K, Yoshimura R, Ohmori Y, Yoshimura N.: "The Role of Tissue Factor in Renal Ischemic Reperfusion Injury of the Rat"JOURNAL OF SURGICAL RESEARCH. 102. 102-109 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakamura K, Kadotani Y, Ushigome H, Akioka K, Okamoto M, Ohmori Y, Yaoi T, Fushiki S, Yoshimura R, Yoshimura N: "Antisense Oligonucleotide for tissue factor inhibits hepatic ischemic reperfusion injury"Biochemicai and Biophysicai Research Communications. 297. 433-441 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Matsuyama M, Akioka K, Okamoto M, Kadotani Y, Nakamura K, Ushigome H, Ohmori Y, Yoshimura R, Nakatani T, Yoshimura N: "CONTROL OF RENAL ISCHEMIC REPERFUSION INJURY BY ANTISENSE OLIGODEOXYNUCLEOTIDE OF TISSUE FACTOR"Transplantation Proceeding. (In press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Matsuyama M, Yoshimura R, Akioka K, Okamoto M, Ushigome H, Kadotani Y, Nakatani T, Yoshimura N: "TISSUE FACTOR ANTISENSE OLIGONUCLEOTIDES PREVENT RENAL ISCHEMIC REPERFUSION INJURY"TRANSPLANTATION. (In press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Matsuyama M, Akioka K, Okamoto M, Kadotani Y, Nakamura K, Ushigome H, Ohmori Y, Yoshimura R, Nakatani T, Yoshimura N: "CONTROL OF RENAL ISCHEMIC REPERFUSION INJURY BY ANTISENSE OLIGODEOXYNUCLEOTIDE OF TISSUE FACTOR"Transplantation Proceeding. In press.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Matsuyama M, Akioka K, Okamoto M, Kadotani Y, Ushigome H, Nakamura K, Yoshimura R, Ohmori Y, Yoshimura N: "The Role of Tissue Factor In Rental Ischemic Reper fusion Injury by Antisense Ologodeoxynucleotide"Transplantation proceeding. 34. 1475-1476 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Usigome H, Sano H, Okamoto M, Kadotani Y, Nakamura K, Akioka K, Yoshimura R, Ohmori Y, Yoshimura N: "The Role of Tissue Factor In Rental Ischemic Reper fusion Injury of tha rat"JOURNAL OF SURGICAL RESEARCH. 102. 102-109 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakamura K, Kadotani Y, Usigome H, Akioka K, Okamoto M, Ohmori Y, Yaoi T, Fushiki S, Yoshimura R, Yoshida N: "Antisense Oligonucleotide for tissue factor inhib its hepatic ischemic reperfusion injury"Biochemical and Biophysical Research Communications. 297. 433-441 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Matsuyama M, Yoshimura R, Akioka K, Okamoto M, Usigome H, Kadotani Y, Nakatani T, Yoshimura N: "TISSUE FACTOR ANTISENSE OLIGONUCLEOTIDES PREVENT RENTAL ISCHEMIC PEPERFUSION INJURY"TRANSPLANTATION. In press.
  • Description: 「研究成果報告書概要(欧文)」より