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2001 Fiscal Year Final Research Report Summary

Role of NKT cells in acceptance of pancreatic islet allografts in mice.

Research Project

Project/Area Number 12671189
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionFukuoka University

Principal Investigator

YASUNAMI Yohichi  School of Medicine, Fukuoka University Associate Professor, 医学部, 助教授 (00166521)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Toshinori  Chiba Univ., Sen. of Medicine, Associate, Professor, 大学院・医学研究科, 助教授 (50237468)
Project Period (FY) 2000 – 2001
Keywordsislet transplantation / NKT cells / rejection / tolerance / allotransplantation / donor specific transfusion
Research Abstract

Pancreatic islet transplantation represents a potential treatment for insulir dependent diabetes mellitus. However, the precise cellular and moleculai mechanisms of immune reactions against allogeneic islets remain uncleatfn the present study, we determined whether NKT cells, which have been recently identified as a distinct lymphoid cell lineage, play a role in acceptance of isl allografts in mice treated with donor specific transfusion (DST). For those purposes, Vα14 NKT cell knockout mice are used asNKT cell deficient recipients.
Pancreatic islet transplantation represents a potential treatment for insulir dependent diabetes mellitus. However, the precise cellular and moleculai mechanisms of immune reactions against allogeneic islets remain uncleatfn the present study, we determined whether NKT cells, which have been recently identified as a distinct lymphoid cell lineage, play a role in acceptance of isl allografts in mice treated with donor specific transfusion (DST). For those purposes, Vα14 NKT cell knockout mice are used asNKT cell deficient recipients.
BALB/c islet allografts were accepted in streptozotocin-induced diabeti C57BL/6 mice (wild-type) by DST in conjunction with CTLA4-Ig. In marked contrast, all BALB/c islet allgrafts were rejected with the similar treatment whe NKT cell-deficient mice are used as recipients, indicating that NKT cells an required for the acceptance of islet allografts by DST The molecule(s) of NKT cells associated with the acceptance is currently under investigation by the analysis of real-time PCR on FiCS-sorted NKT cells.
A novel procedure to prevent allograft rejection by means of CTLA4Ig was also investigated.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] T Maki, Y Yasunami, et al.: "Prolongation of rat islet xenograft survival in the liver of IFN-γ-deficient mice"Journal of Surgical Research. 93. 101-107 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M Nakano, Y Yasunami: "Can hepatocyte growth factor have an effect on regeneration of islet grafts?"Transplantation. 71. 820-821 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Megumi Takehara, et al.: "Long-term acceptance of allografts by invivo gene transfer of regulatable adenovirus vector containing CTLA4Ig and IoxP"Human Gene Therapy. 12. 415-426 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T Shiraishi, Y Yasunami, et al.: "Prevention of acute rat lung allograft rejection in rat by CTLA4Ig"American Journal of Transplantation. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maki T, Yasunami Y, Ikehara Y, Kodama S, Nakano M, Nakayama T, Taniguchi M, Ikeda S: "Prolongation of rat islet xenograft survival in the liver of IFN- γ -deficient mice"J Surg Res. 93(1). 101-107 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakano M, Yasunami Y: "Can hepatocyte growth factor have an effect on regeneration of islet grafts"Transplantation. 71. 820-821 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takehara Megumi, Murakami Masaaki, Inobe Manabu, Tanaka Kumiko, Chikuma Shunsuke, Saito Izumi, Kanegae Yumi, Yasunami Yohichi, Nakano Masahiko, Yamashita Kenichiro, Todo Satoru, Uede Toshimitsu: "Long-term acceptance of allografts by in vivo gene transfer of regulatable adenovirus vector containing CTLA4Ig and loxF"Human Gene Therapy. 12. 415-426 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiraishi T, Yasunami Y, Takehara M, Kawahara K, Shirakusa T: "PrevenliQn of acute rat lung allograft rejection in rat by CTLA4Ig"Am J Transplant. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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