2001 Fiscal Year Final Research Report Summary
Role of NKT cells in acceptance of pancreatic islet allografts in mice.
| Project/Area Number |
12671189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Fukuoka University |
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Principal Investigator |
YASUNAMI Yohichi School of Medicine, Fukuoka University Associate Professor, 医学部, 助教授 (00166521)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Toshinori Chiba Univ., Sen. of Medicine, Associate, Professor, 大学院・医学研究科, 助教授 (50237468)
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| Project Period (FY) |
2000 – 2001
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| Keywords | islet transplantation / NKT cells / rejection / tolerance / allotransplantation / donor specific transfusion |
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| Research Abstract |
Pancreatic islet transplantation represents a potential treatment for insulir dependent diabetes mellitus. However, the precise cellular and moleculai mechanisms of immune reactions against allogeneic islets remain uncleatfn the present study, we determined whether NKT cells, which have been recently identified as a distinct lymphoid cell lineage, play a role in acceptance of isl allografts in mice treated with donor specific transfusion (DST). For those purposes, Vα14 NKT cell knockout mice are used asNKT cell deficient recipients.
Pancreatic islet transplantation represents a potential treatment for insulir dependent diabetes mellitus. However, the precise cellular and moleculai mechanisms of immune reactions against allogeneic islets remain uncleatfn the present study, we determined whether NKT cells, which have been recently identified as a distinct lymphoid cell lineage, play a role in acceptance of isl allografts in mice treated with donor specific transfusion (DST). For those purposes, Vα14 NKT cell knockout mice are used asNKT cell deficient recipients.
BALB/c islet allografts were accepted in streptozotocin-induced diabeti C57BL/6 mice (wild-type) by DST in conjunction with CTLA4-Ig. In marked contrast, all BALB/c islet allgrafts were rejected with the similar treatment whe NKT cell-deficient mice are used as recipients, indicating that NKT cells an required for the acceptance of islet allografts by DST The molecule(s) of NKT cells associated with the acceptance is currently under investigation by the analysis of real-time PCR on FiCS-sorted NKT cells.
A novel procedure to prevent allograft rejection by means of CTLA4Ig was also investigated.
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[Publications] Takehara Megumi, Murakami Masaaki, Inobe Manabu, Tanaka Kumiko, Chikuma Shunsuke, Saito Izumi, Kanegae Yumi, Yasunami Yohichi, Nakano Masahiko, Yamashita Kenichiro, Todo Satoru, Uede Toshimitsu: "Long-term acceptance of allografts by in vivo gene transfer of regulatable adenovirus vector containing CTLA4Ig and loxF"Human Gene Therapy. 12. 415-426 (2001)
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