2001 Fiscal Year Final Research Report Summary
Analysis of colon-specific unknown gene possibly associated with inflammatory bowel disease
| Project/Area Number |
12671194
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
SHIBATA Chikashi Tohoku Univ. Hospital, Research Associate, 医学部・附属病院, 助手 (30270804)
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| Co-Investigator(Kenkyū-buntansha) |
FUNAYAMA Yuji Tohoku Univ. Hospital, Lecturer, 医学部・附属病院, 講師 (50192315)
SASAKI Iwao Tohoku Univ. Graduate School of Med., Professor, 大学院・医学系研究科, 教授 (60125557)
FUKUSHIMA Kouhei Tohoku Univ. Graduate School of Med., Research Associate, 大学院・医学系研究科, 助手 (20271900)
NAITO Hiroo Tohoku Univ. Hospital, Lecturer, 医学部・附属病院, 講師 (90180223)
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| Project Period (FY) |
2000 – 2001
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| Keywords | intestinal flora / epithelial cells / syncollin / host defense / exocytosis |
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| Research Abstract |
Background arid Aims : Intestinal microorganisms play role in inflammatory bowel disease. The aim of this study was to identify epithelial gene expression down-regulated by bacterial flora, and to examine expression of human homologue and possible role in minflammatory bowel disease.
Methods : Germ-free mice were orally given bacterial suspension prepared from conventional components (bacterial reconstitution), and differential gene expression in intestinal epithelial cells was identified by differential display. Expression of the identified gene was examined in dextran sulfate-sodium induced colitis or human inflammatory bowel disease, by Northern blot and quantitative RT-PCR.
Results : Syncollin mRNA was identified as a strongly down-regulated gene after bacterial reconstitution. In contrast, syncollin mRNA expression was stable in colonic epithelial cells of dextran sulfate-sodium -treated mice. Human syncollin mRNA deceased in UC but not CD epithelial cells in an independent manner of macroscopic inflammation.
Conclusion : Since rat syncollin is an essential molecule in exocytotic pathway, decreased syncollin expression in UC may be associated with abnormal secretion.
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