2001 Fiscal Year Final Research Report Summary
Specific CTL Activity of Lamina Propria Lymphocytes in Idiopathic Inflammatory Bowel Diseases.
| Project/Area Number |
12671217
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Gifu University |
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Principal Investigator |
NITTA Toyoo Gifu Univ. Hospital, Assistant, 医学部附属病院, 助手 (70313905)
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| Co-Investigator(Kenkyū-buntansha) |
HIROSE Hajime Gifu Univ. Professor, 医学部, 教授 (20101272)
IWATA Hisashi Gifu Univ. Hospital, Instructor, 医学部附属病院, 講師 (90303495)
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| Project Period (FY) |
2000 – 2001
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| Keywords | idiopathic inflammatory bowel disease / 2,4,6-Trinitrobenzene-sulfonic acid(TNBS) induced colitis / cytotoxicity / TCR / V β repertoire |
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| Research Abstract |
In this study, We analyzed the functional role of CD8^+TCRV β 14T-cells in 2,4,6-Trinitrobenzene-sulfonic acid(TNBS) induced colitis as follows ;
1. ^<51>Cr release assay : CD8^+TCR V β 14 T-cell clones showed specific cytotoxic activity against TNBS conjugated self spleen cells.
2. ELISA : These clones produced IFN-γ in culture supernatant, but neither IL-2 nor IL-4.
3. Adoptive transfer : Recipient animals transferred with these clones developed colitis when exposed to low doses of TNBS.
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