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2002 Fiscal Year Final Research Report Summary

Establishment of hepatic reserve function and molecular biological analysis of liver function after hepatectomy

Research Project

Project/Area Number 12671235
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionMiyazaki Medical College (2002)
Kyushu University (2000-2001)

Principal Investigator

CHIJIIWA Kazuo  Miyazaki Medical College, Surgery 1, Chairman and Professor, 医学部, 教授 (90179945)

Co-Investigator(Kenkyū-buntansha) MAKINO Ichiro  Miyazaki Medical College, Surgery 1, Assistant Professor, 医学部, 助手 (30347059)
KAI Masahiro  Miyazaki Medical College, Surgery 1, Assistant Professor, 医学部, 助手 (40264379)
KONDO Kazuhiro  Miyazaki Medical College, Surgery 1, Assistant Professor, 医学部, 助手 (10244196)
UEDA Junji  Miyazaki Medical College, Surgery 1, Medical Staff, 医学部, 医員
Project Period (FY) 2000 – 2002
KeywordsObstructive jaundice / Liver cirrhosis / Biliary drainage / Liver regeneration / Cyclin / Cyclin dependent kinase / Cell cycle / Transcriptional factor
Research Abstract

1) We investigated the differences in the transcription factors and cell cycle regulators between obstructive jaundiced and control rats before and after hepatectomy. The expressions and activities of C/EBP-α and ?β were significantly decreased associated with the significantly lower cyclin E expression after hepatectomy in the jaundiced group than in the controls. The activities of AP-1, cyclin D1, and p21 were not significantly different between the two groups. These results suggest that obstructive jaundice inhibits hepatic expression and activities of C/EBP, resulting in the impaired cyclin E expression that is partly responsible for the cell cycle dysfunction after hepatectomy. (Reference 1)
2) Regeneration responses in growth factor receptors, transcription factors, and cell cycle regulators after two-third hepatectomy were compared between rats with thioacetamide-induced cirrhotic and normal liver. The activities of C/EBP and AP- 1 were significantly inhibited in the cirrhotic gr … More oup compared with those in the control group, but not those of NF-kB and STAT 3. The expression of cyclin D1, E, and A was significantly decreased in the cirrhotic group compared with the control group. These results suggest that downregulation of cyclin D1, E, and A expression, which may be induced by impaired activities of C/EBP and AP-1, is responsible for the decreased regenerative capacity of cirrhotic liver after partial hepatectomy. (Reference 2)
3) The importance of bile in liver regeneration after hepatectomy is known. Bile was drained externally (ED group) or into the duodenum (ID group) for 7 days before 70% hepatectomy, and liver regeneration was assessed by the relative liver weight, DNA synthesis rate, and PCNA labeling index. Posthepatectomy expressions of cyclin D1 and E, and cyclin D1- and E-associated kinase activity were also analyzed. Liver regeneration in the ED group was delayed than that in the ID group. Cyclin D1 and E expression and cyclin D1-associated kinase activity were not different between the two groups. Cyclin E-associated kinase activity was lower in the ID group than the ED group at 18 hr after hepatectomy. These results suggest the absence of intestinal bile delays liver regeneration associated with cyclin E-associated kinase inactivation after hepatectomy. (Reference 3) Less

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Zhao G, Nakano K, Chijiiwa K, et al.: "Inhibited activities in CCAAT/enhancer-binding protein, activating protein-1, and cyclins after hepatectomy in rats with thioacetamide-induced cirrhosis"Biochemical and Biophysical Research Communications. 292・2. 474-481 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ueda J, Chijiiwa K, et al.: "Lack of intestinal bile results in delayed liver regeneration of normal rat liver after hepatectomy accompanied by impaired cyclin E-associated kinase activity"Surgery. 131・5. 564-573 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mizuta A, Chijiiwa K, et al.: "Differences in biliary lipid excretion after major hepatectomy in obstructive jaundiced rats with preoperative internal, external, or no biliary drainage"European Surgical Research. 34・4. 291-299 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chijiiwa K, et al.: "Serum interleukin-6 and hepatocyte growth factor levels in patients after hepatectomy"Hepatogastroenterology. 49・44. 467-471 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chijiiwa K, et al.: "Relation of biliary bile acid output to hepatic adenosine triphosphate level and biliary indocyanine green excretion in humans"World Journal of Surgery. 26・4. 457-461 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe M, Chijiiwa K, et al.: "Effects of streptococcal preparation OK-432 on liver regeneration and energy status after partial hepatectomy under ischemia/reperfusion in rats"Hepatogastroenterology. 49・43. 218-221 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe M, Chijiiwa K, et al.: "FR167653 improves survival and pulmonary injury after partial hepatectomy under ischemia/reperfusion in rats"Journal of Surgical Research. 101・2. 146-151 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakano K, Chijiiwa K, et al.: "Lower activity of CCAAT/enhancer-binding protein and expression of cyclin E, but not cyclin D1, activating protein-1 and p21(WAF1), after partial hepatectomy in obstructive jaundice"Biochemical and Biophysical Research Communications. 280・3. 640-645 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chijiiwa K, et al.: "Effect of preoperative portal vein embolization on liver volume and hepatic energy status of the nonembolized liver lobe in humans"European Surgical Research. 32・2. 94-99 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe M, Chijiiwa K, et al.: "Gadolinium pretreatment decreases survival and impairs liver regeneration after partial hepatectomy under ischemia/reperfusion in rats"Surgery. 127・4. 456-463 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chijiiwa K, et al.: "Biliary indocyanine green excretion as a predictor of hepatic adenosine triphosphate levels in patients with obstructive jaundice"American Journal of Surgery. 179・2. 161-166 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakano K, Chijiiwa K, Tanaka M: "Lower activity of CCAAT/enhancer-binding protein and expression of cyclin E, but not cyclin D1, activating protein-1 and p21 (WAF1), after partial hepatectomy in obstructive jaundice."Biochem Biophys Res Commun. 280(3). 640-645 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zhao G, Nakano K, Chijiiwa K, Ueda J, Tanaka M: "Inhibited activities in CCAAT/enhancer-binding protein, activating protein-1 and cyclins after hepatectomy in rats with thioacetamide-induced liver cirrhosis."Biochem Biophys Res Commun. 292(2). 474-481 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda J, Chijiiwa K, Nakano K, Zhao G, Tanaka M: "Lack of intestinal bile results in delayed liver regeneration of normal rat liver after hepatectomy accompanied by impaired cyclin E-associated kinase activity."Surgery. 131(5). 564-573 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mizuta A, Chijiiwa K, Saiki S, Kuroki S, Nakamura K, Tanaka M: "Differences in biliary lipid excretion after major hepatectomy in obstructive jaundiced rats with preoperative internal, external, or no biliary drainage."Eur Surg Res. 34(4). 291-299 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chijiiwa K, Saiki S, Tanaka M: "Serum interleukin-6 and hepatocyte growth factor levels in patients after hepatectomy."Hepatogastroenterology. 49(44). 467-471 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chijiiwa K, Mizuta A, Ueda J, Takamatsu Y, Nakamura K, Watanabe M, Kuroki S, Tanaka M: "Relation of biliary bile acid output to hepatic adenosine triphosphate level and biliary indocyanine green excretion in humans."World J Surg. 26(4). 457-461 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe M, Chijiiwa K, Kameoka N, Nakano K, Noshiro H, Tanaka M: "Effects of streptococcal preparation OK-432 on liver regeneration and energy status after partial hepatectomy under ischemia/reperfusion in rats."Hepatogastroenterology. 49(43). 218-221 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe M, Yamaguchi K, Chijiiwa K, Tanaka M: "FR167653 improves survival and pulmonary injury after partial hepatectomy under ischemia/reperfusion in rats."J Surg Res. 101(2). 146-151 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chijiiwa K, Saiki S, Noshiro H, Kameoka N, Nakano K, Tanaka M: "Effect of preoperative portal vein embolization on liver volume and hepatic energy status of the nonembolized liver lobe in humans."Eur Surg Res. 32(2). 94-99 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe M, Chijiiwa K, Kameoka N, Yamaguchi K, Kuroki S, Tanaka M: "Gadolinium pretreatment decreases survival and impairs liver regeneration after partial hepatectomy under ischemia/reperfusion in rats."Surgery. 127(4). 456-463 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chijiiwa K, Watanabe M, Nakano K, Noshiro H, Tanaka M: "Biliary indocyanine green excretion as a predictor of hepatic adenosine triphosphate levels in patients with obstructive jaundice."Am J Surg. 179(2). 161-166 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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