2002 Fiscal Year Final Research Report Summary
I densification of genes regulating colorectal carcwageuesis by using the algorithm for disguising malignant state method
| Project/Area Number |
12671244
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
ISHIKAWA Takashi Yokohama City University, School of Medicine, Assistant professor, 医学部, 助手 (80275049)
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| Co-Investigator(Kenkyū-buntansha) |
OKAZAKI Yasushi Rlken Institute, Team Leader, 理化学研究所, チームリーダー (80280733)
HAYASHIZAKI Yoshihide Rlken Institute, Project Header, 理化学研究所, プロジェクトリーダー (70192705)
ICHIKASA Yasushi Yokohama City University, School of Medicine, Assistant Professor, 医学部, 助手 (70254208)
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| Project Period (FY) |
2000 – 2002
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| Keywords | cDNA micro array / colon cancer / gene expression profile / date analysis |
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| Research Abstract |
We studied the expression profiles of various stages of colorectal tumors (adenoma (AD), seven samples; carcinoma (CA), 16 samples) by using cDNA micro arrays and developed ADMS (algorithm for diagnosing malignant state) method, selecting 335 clones characteristic of CA state. We, then, applied ADMS to 12 additional samples (five from primary lesions with metastasis and seven metastases); all 16 Cas and 12 metastasis tumors were diagnosed correctly as cancerous states. Although three of the seven Ads were diagnosed as "cancerous", the large size of two of these tumors suggested their potential malignancy. Our strategy for selecting clones characteristic of the malignant state is widely applicable to diagnosis and for predicting the stage of progression during multistep carcinogens. Of the 335 clones we selected, 135 were known genes. Included in the 135 genes were tumor suppressor and growth factor-related genes and were consistent with the literature. ADMS is a reliable means for identifying genes useful for the diagnosis of cancer.
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