2001 Fiscal Year Final Research Report Summary
Development of viral antigen specific immunotherapy for Epstein-Barr virus related gastric cancer.
| Project/Area Number |
12671249
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
UEDA Yuji Kyoto Prefectural University of Medicine, Digestive Surgery, Assistant Professor., 医学部, 助手 (60254356)
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| Co-Investigator(Kenkyū-buntansha) |
竹迫 一迫 宝酒造, バイオ研究所, 副所長
YAMAGISHI Hisakazu Kyoto Prefectural University of Medicine, Digestive Surgery, Professor., 医学部, 教授 (40128723)
MATSUDA Osamu Kyoto Prefectural University of Medicine, Digestive Surgery, Assistant Professor., 医学部, 助教授 (00271164)
TAKESAKO Kazutoh Takara Shuzo, Biotechnology Laboratories, Chief Researcher
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| Project Period (FY) |
2000 – 2001
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| Keywords | EBV (Epstein-Barr Virus) / Gastric Cancer / Tumor Antigen Peptide / Tumor Specific Immunotherapy / Cancer vaccine Therapy / LMP2(Latent Membrane Protein 2) |
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| Research Abstract |
(1) Cytotoxic T cell(CTL) induction with latent membrane protein 2(LMP2) epitope peptide -loaded dendritic cells(DCs).
We synthesized the LMP2 peptide, TYGPVFMCL(HLA-A24 restricted), and established CTL line by stimulating PBMCs from HLA-A24 positive healthy donors in vitro with LMP2 peptide-loaded DCs. This CTL could lyse the target cells pulsed with this peptide, but couldn't lyse LMP2-CDNA transformed target cells.
(2) CTL induction with autologous EBV transformed B cell line(LCL).
In order to establish LCL, concentrated supernatant from B95-8 cultures, a human type1 EBV-transformed marmoset B-cell line, was added to a healthy donor's PBMCs. PBMCs from healthy donors were stimulated in vitro with autologous LCL. This LCL-induced CTL recognized the full repertoire of EBV latency antigens. We selected LMP2-specific CTL line after 5lCr release assay.
(3) Detection of EBV-DNA by polymers chain reaction (PCR).
In order to detect EBV related gastric cancers from patients underwent gastorectomy in our institute, we performed PCR and southern hybridization. After that, we tried to establish LMP2 positive cell lines of gastric cancer which could be useful targets for CTL-based immunotherapy.
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