2003 Fiscal Year Final Research Report Summary
A new method for detection of gene mutations using both E.coli MutS protein and resolvase
| Project/Area Number |
12671256
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | JICHI MEDICAL SCHOOL |
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Principal Investigator |
SHITOH Kazuhisa Jichi Medical School of Medicine, Assistant professor, 医学部, 助手 (90285792)
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| Co-Investigator(Kenkyū-buntansha) |
TSUKAMOTO Toshihiko Kitasato University, Assistant Professor, 薬学部, 助教授 (10236862)
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| Project Period (FY) |
2000 – 2003
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| Keywords | E.coli Muts / Mismatch repair genes / Hereditary nonpolyposis colorectal carcinoma (HNPCC) / hMLH1 / hMSH2 / Japanese clinical criteria / hMLH1 methylation / BRAF |
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| Research Abstract |
We have investigated to upgrade the detection efficiency for gene mutation modifying both E.coli Muts and resolvase. Together with it, we analyzed 452 colorectal carcinoma cases, of which 69 cases fulfilled the Japanese clinical criteria and 106 fulfilled the Bethesda guidelines to evaluate the better method for detection of HNPCC (hereditary nonpolyposis colorectal carcinoma). As results, Japanese clinical criteria A are suitable for selecting cases to analyze for gene mutations. And we suggested that an age at onset younger than 50 years was also important for screening. Analyzing TGF β RII mutations and immunohistochemical staining of hMLH1 and hMSH2 for cases with MSI (microsatellite instability) phenotype are useful for selecting cases who should be tested for germline mutations.
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[Publications] Koinuma K, Shitoh K, Miyakura Y, Furukawa T, Yamashita Y, Ota J, Ohki R, Choi YL, Wada T, Konishi F, Nagai H, Mano H: "Mutations of BRAF are associated with extensive hMLM1 promoter methylation in sporadic colorectal carcinoma."Int J Cancer. 108. 237-242 (2004)
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