2001 Fiscal Year Final Research Report Summary
Apoptosis in the acute rejection of cardiac Xenotransplantation
| Project/Area Number |
12671293
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Fukushima Medical University school of Medicine |
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Principal Investigator |
HITOSHI Yokoyama Fukushima Medical University school of Medicine, Professor, 医学部, 教授 (80282127)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASHI 0no Fukushima Medical University school of Medicine, Assistant, 医学部, 助手 (30274955)
HIRONO Satokawa Fukushima Medical University school of Medicine, Lecturer, 医学部, 講師 (70226025)
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| Project Period (FY) |
2000 – 2001
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| Keywords | xenotransplantation / apoptosis / acute rejection |
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| Research Abstract |
[Aim] To clarify the relationship between cardiac xenograft rejection and apoptosis using experimental heart transplantation model (Hamster to Rat)
[Method] Experimental cardiac Xenotransplantation was performed using Hamster as the heart donor and Lewis rat as the recipient with the modified method described by Ono and Lindsey. Transplanted heart were examined in 0, 4, 8, 24 and 48 hours after transplantation.
[Results] Mean survival days for the transplanted xenograft was 3 days. Hematoxylin- eosin staining of the myocardium revealed no particular change during 4-8 hours after Tx, and neutrophylic cell infiltration around vessels after 24 hours. Focal infiltration of the neutrophils was observed in the epicardium and endocardium after 48 hours. Myocardial necrosis was observed after 48 hours. No vasculitis was observed after 48 hours. Lymphocyte infiltration was not obvious during all rejection process. TUNEL. Staining revealed positive cell nucleus of endocardium and vascular endothel during 4 to 48 hours after Tx. Fas positive cells were observed in the endocardium and vascular endothelium during 4 to 8 hours after Tx.
[Conclusions] Neutrophilic cells are main infiltration cells during concordant cardiac xenograft rejection, which is different with allograft rejection with lymphocyte infiltration. Fas associated apoptosis was in process on the endocardium and vascular endothelium before morphological rejection, which suggests suppression of apoptosis might lead to concordant cardiac xenograft rejection.
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