Angiogenesis in ischemic myocardium using electroporation method

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 12671320
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Yokohama City Univ.
• Principal Investigator: ISODA Susumu School of medicine, Yokohama City Univ., Assist. Prof., 医学部・附属病院, 講師 (40254172)
• Co-Investigator(Kenkyū-buntansha): YANO Yoshimi School of medicine, Yokohama City Univ., Assistant, 医学部・附属病院, 助手 (00315772) ; TOBE Michio School of medicine, Yokohama City Univ., Assist. Prof., 医学部, 講師 (70244431)
• Project Period (FY): 2000 – 2001
• Keywords: ischemic myocardium / VEGF / electroporation / angiogenesis

Research Abstract

Main therapy to ischemic myocardium include medical, interventional, and surgical one. Transmyocardial revascularization(TMR) is also studied but is still adjunctive. Angiogenesis therapy using FGF, VEGF, HGF and their gene was introduced in 1990. We focused on the technique of electroporation method which was successful in efficiency improvement of gene transfer in the field of plants. We used electroporation technique and plasmid injection in the muscle to treat the ischemic legs of rat. We proved expression of the VEGF, angiogenesis occurrence, and improvement of leg blood perfusion. We applied the technique to treat ischemic myocardium.