2001 Fiscal Year Final Research Report Summary
Non-invasive measurement of cerebral inhibitory receptors and their neural circuit in epileptgenic
| Project/Area Number |
12671343
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NARIAI Tadashi Tokyo Medical and Dental University, Medical Hospital, Lecturer, 医学部・附属病院, 講師 (00228090)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIWATA Kiichi Tokyo Metropolitan Institute of Gerontology, Senior Staff, ポジトロン医学研究部門, 主任研究員 (50143037)
TANAKA Yoji Tokyo Medical and Dental University, Medical Hospital, Research associate, 医学部・附属病院, 助手 (80323682)
OHTA Yoshihisa Tokyo Medical and Dental University, Medical Hospital, Research Associate, 医学部・附属病院, 助手 (70311652)
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| Project Period (FY) |
2000 – 2001
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| Keywords | adenosine receptor / benzodiazepine receptor / positron emission tomography / temporal lobe epilepsy / hippocampus / cerebral glucose metabolism / C-11 MPDX / kinic acid |
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| Research Abstract |
1) All the patients with intractable epilepsy who were operated on in Department of Neurosurgery, Tokyo Medical and Dental University underwent PET measurement of cerebral metabolic rate of glucose and the binding potential, of central benzodiazepine receptors. In 2001, a new project to image cerebral histamine receptor in epileptic foci with C-11 doxepine and PET was started. The results are ready to be analized with kinetic analysis of receptor binding.
2) We have been developing a novel receptor ligand to map cerebral adenosine receptor system. Through this project, C-11 MPDX was recognized as a suitable ligand to image A1 receptor in vivo condition with PET. Pre-clinical research to establish the efficacy of this tracer was conducted with cat and monkey and enough data to indicate the usefulness and safetiness of this tracer was accumulated. Based on this data, PET imaging of adenosine A1 receptor in human brain will be started in next fiscal year.
3) As described above, measurement of benzodiazepine and adenosine receptor system in human brain is and will be possible with PET. To confirm the contribution of these two receptor system in epileptogenicity, in vitro autoradiographical experiment was conducted with rats model of limbic seizure. Increased expressinon of these receptors was recognized in hippocampus of epilepsy rats.
4) A method to investigate the cerebral metabolism and receptor binding using incubated living brain slice was developed using positron tracer. A new project to apply this technique to human brain specimen obtained from epilepsy patients was started.
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[Publications] Sato, K., T. Nariai, S. Sasaki, I. Yazawa, H. Mochida, N. Miyakawa, Y. Momose-Sato, K. Kamino, Y. Ohta, K. Hirakawa, and K. Ohno: "Intraoperative Intrinsic Optical Imaging of Neuronal Activity from Subdivisions of the Human Primary Somatosensory Cortex"Cereb Cortex. 12(3). 269-280 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nriail, T, Ohta, Y, Hirakawa, K, Sato, K, Yazawa, I, Sasaki, S, Kamino, K, Ohno, K.: "Intrinsic Optical recording of somatosensory responses in the human cerebral cortex during brain tumor surgery"Tomita M et al. Eds. International Congress Series. Elsevier Science 2002 (in press).
Description
「研究成果報告書概要(欧文)」より
