2001 Fiscal Year Final Research Report Summary
Effect of CSF with subarachnoid hemorrhage on cerebral smooth muscle cells
| Project/Area Number |
12671385
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | Toho University School of Medicine |
|---|
Principal Investigator |
IWABUCHI Satoshi Toho University School of Medicine, 2nd. Department of Neurosurgery, Assistant Professor, 医学部, 講師 (30213309)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
AOKI Kazuya Toho University School of Medicine, 2nd. Department of Neurosurgery, Instructor, 医学部, 助手 (50202461)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Keywords | subarachnoid hemorrhage (SAH) / vasospasm / vascular smooth muscle cell / intracellular free Ca^<2+> concentration / nucleotide / protein tyrosine kinase (PTK) / mitogen activated protein kinase (MAPK) |
|---|
| Research Abstract |
We investigated the effect of cerebrospinal fluid (CSF) following subarachnoid hemorrhage (SAH) on isolated smooth-muscle cells. The application of CSF obtained on day 3 following SAH induced the most contraction. An increase in the intracellular Ca^<2+> concentration of smooth-muscle cells was observed during contraction. There is a discrepancy between this observation and the peak of clinical vasospasm occurring about 7 days after SAH. Following SAH, CSF may take a few days to affect smooth-muscle cells in the media of cerebral arteries. Preincubation of cells with suramin, an antagonist of P2x and P2y purinoceptors, attenuated the contraction after application of CSF, suggesting that nucleotides may be involved in the contraction of vascular smooth-muscle cells after SAH.
We also investigated the role of mitogen-activated protein kinase (MAPK) and protein tyrosine kinase (PTK) in mediating ATP-induced Ca^<2+> mobilization in rat basilar artery smooth muscle cells. Extracellular ATP produced intracellular free Ca^<2+> elevation and MAPK phosphorylation in smooth muscle cells, and regulated by PTK. In the study using canine double hemorrhage model of SAH, MAPK immunoprecipitaion was significantly enhanced in the spastic arteries. The peak immunoprecipitaion occurred on day 3 after blood injection. MAPK kinase inhibitor PD98059 attenuated the MAPK immunoprecipitaion and vasoconstriction. PD98059 might be useful in the management of cebral vasospasm.
|
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Publications] Kawasaki C, Iwabuchi S, Kasai K, Yokouchi T, Ito K, Kubo T, Yoshii S, Sugiura E, Tomiyama A, Hayashi M, Ushikubo Y, Ueda M, Samejima H: "Evaluation of vasospasm following subarachnoid hemorrhage in the deffusion-weighted MR imaging and SPECT"Proc Spasm Symp.. Vol. 15. 136-44 (2000)
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Publications] Yokouchi T, Iwabuchi S, Hayashi M, Izukura H, Ito K, Kawasaki C, Kusaka G, Kasai K, Ushikubo Y, Ueda M, Samejima H: "Evaluation of intra-arterial infusion of fasudil hydrochloride for the treatment pf angiographic cerebral vasospasm"Proc Spasm Symp.. Vol. 16. 316-20
Description
「研究成果報告書概要(欧文)」より
