2002 Fiscal Year Final Research Report Summary
THE ROLE OF NITRIC OXIDE DURING THE PROCESS OF INTERVERTEBRAL DISC DEGENERATION AND PREVENTION OF DISC DEGENERATION USING i-NOS ANTI SENSE OLIGONUCLEOTIDE
| Project/Area Number |
12671401
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY |
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Principal Investigator |
ISHIHARA Hirokazu Toyama Medical and Pharmaceutical University, University Hospital, Assistant Professor, 附属病院, 講師 (30242499)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAGUCHI Yoshiharu Toyama Medical and Pharmaceutical University, Faculty of Medicine, Instructor, 医学部, 助手 (00262527)
OSADA Ryusuke Toyama Medical and Pharmaceutical University, University Hospital, Instructor, 附属病院, 助手 (40293310)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Nitric Oxide / Proteoglycan / Intervertebral disc metabolism / i-NOS / アンチセンスオリゴヌクレオチド |
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| Research Abstract |
The intervertebral disc degeneration is the major cause of disc dysfunction and low back pain. Many factors are known to influence intervertebral disc degeneration. Nitric Oxide (NO) is an inorganic, gaseous free radical and identified as the elusive blood vessel relaxing factor. We have clarified that the role of NO in disc metabolism and the effects of i-NOS anti-sense oligonucleotide that control NO production to prevent experimental rabbit disc degeneration model. The degenerated rabbit intervertebral disc spontaneously produced NO and NO inhibited proteoglycan synthesis and increased the production of Matrix metalloproteinase-3 in the disc. I-NOS anti-sense oligonucleotide inhibited proteoglycan loss and matrix metalloproteinase production.
Histologically, i-NOS anti-sense oligonucleotide inhibited the reduction of Safranin-O staining and production of annulus fibrosus tear during the degeneration process.
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