2001 Fiscal Year Final Research Report Summary
Investigation for the effect of growth hormone and thyroid hormone on growth factors in bones and cartilages
| Project/Area Number |
12671436
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kitasato University |
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Principal Investigator |
MINEHARA Hiroaki Kitasato Univ. School of Medicine Research Associate, 医学部, 助手 (70327352)
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| Co-Investigator(Kenkyū-buntansha) |
UCHINO Masataka Kitasato Univ. School of Medicine Research Associate, 医学部, 助手 (90255323)
URABE Ken Kitasato Univ. School of Medicine Assistant Professor, 医学部, 講師 (90284489)
ITOMAN Moritoshi Kitasato Univ. School of Medicine Professor, 医学部, 教授 (70104528)
MIYABE Motoi Kitasato Univ. School of Medicine Research Associate, 医学部, 助手 (20296487)
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| Project Period (FY) |
2000 – 2001
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| Keywords | epiphyseal cartilage / thyroid hormone / growth hormone / growth factor / fracture healing / osteoarthritis |
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| Research Abstract |
1) We observed the effect of growth hormone and thyroid hormone using rat femur organ culture. Our studies indicate that the effect of growth hormone and thyroid hormone as local factors might have the reaction through some growth factors other than direct effect.
2) Callus formation is important on fracture healing. To investigate the effect of fixation instability on the hard and soft callus, the morphological changes in fracture callus were studied using a rat femur fracture model. A stainless steal pin or a nylon filament was used for internal fixation, and compared this to a group without fixation. Proliferating cells and TGF-β1 expression in fracture callus were examined by immunohistochemistry. Our studies suggest that greater instability causes more connective tissue generation associated with vigorous cell proliferation, resulting in enlarged cartilage tissue. However, excessive inter fragmentary movement at the fracture site suppress chondrocyte differentiation, and this is associated with predominant TGF-β1 expression.
3) To investigate the effect of growth factors on osteophytes in osteoarthritis (OA), we observed the expression of growth factors by RT-PCR and immunohistochemistry using osteophytes on femoral head during operation for total hip arthroplasty. We also used the femoral head cancellous bone with osteoarthritis or aseptic necrosis (AN) as the sample. TGF-β1 mRNA expression was seen in the surface of osteophytes and connective tissues intracellularly. B-FGF mRNA expression was seen in the deeper layer of osteophytes. On the other hand, expression was not seen in cancellous bone of OA and AN. B-FGF expression was more detected than TGF-β1 in the cartilage with degeneration in OA. These results suggest that TGF-β1 and b-FGF are involved in the formation of osteophytes. Different localization indicates that TGF-β1 seem to have stronger influence.
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[Publications] Ohtsuka, H., Yokoyama, K., Tonegawa, M., Higashi, K., Itoman, M.: "Technique for removing a bent intramedullary femoral nail"J. Orthop.Trauma, May. 15(4). 299-301 (2001)
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[Publications] Yokoyama, K., Itoman, M., Nakamura, K., Tsukamoto, T., Saita, Y., Aoki, S.: "Free vascularized fibular graft vs. Ilizarov method for Post-traumatic tibial bone defect"J. Reconstr. Microsurg, Jan. 17(1). 17-25 (2001)
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[Publications] Takahira, N., Shindo, M., Tanaka, K., Nishimaki, H., Ohwada, T., Itoman, M.: "Gluteal muscle necrosis following transactheter angiographic embolisation for retroperitoneal haemorrhage associated with pelvic fracture"Injury, jan. 32(1). 27-32 (2001)
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