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2002 Fiscal Year Final Research Report Summary

Intracellular mechanism of opioids on myocardial contraction

Research Project

Project/Area Number 12671487
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionSapporo Medical University School of Medicine

Principal Investigator

KANAYA Noriaki  Sapporo Medical University School of Medicine, Anesthesiology, Assistant Professor, 医学部, 講師 (10244344)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Masayasu  Sapporo Medical University School of Medicine, Anesthesiology, Instructor, 医学部, 助手 (60336401)
Project Period (FY) 2000 – 2002
KeywordsHeart / Opioid / calcium
Research Abstract

Freshly isolated rat ventricular myocytes were loaded with the Ca^<2+> indicator, fura-2, at 37℃ for 20 min. Intracellular Ca^<2+> transients and myocyte shortening were simultaneously monitored. Addition of fentanyl (30-1000 nM) resulted in dose-dependent decreases in both peak Ca^<2+>, and myocyte shortening. Morphine (3-100 μM) caused a decrease in myocyte shortening without any concomitant decreases in Ca^<2+> transient. Morphine, but not fentanyl, decreased the amount of Ca^<2+> released from intracellular stores in response to caffeine. Both fentanyl (100 nM) and morphine (10 μM) showed a rightward shift in the dose-response curve to extracellular Ca^<2+> for shortening, with no concomitant effect on the intracellular Ca^<2+> transient. These results indicate that fentanyl and morphine have a direct negative inotropic effect on cardiac excitation contraction coupling at the cellular level. Fentanyl, but not morphine, causes a myocardial depression which is mediated by a decrease in the availability of intracellular free Ca^<2+>. Fentanyl may also alter sarcoplasmic reticulum Ca^<2+> handling and decrease myofilament Ca^<2+> sensitivity, as suggested by changes in the timing of the contractile parameters and the rightward shift in the dose-response curve to extracellular Ca^<2+>, respectively. In contrast, morphine causes a myocardial depression mainly mediated by a decrease in myofilament responsiveness to Ca^<2+>.

  • Research Products

    (8 results)

All 2002 2001

All Journal Article (7 results) Book (1 results)

  • [Journal Article] Propofol anesthesia enhances the pressor response to intravenous ephedrine.2002

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesth Analg 94

      Pages: 1207-1211

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Differential effects of midazolam and diazepam on intracellular Ca^<2+> transients and contraction in adult rat ventricular myocytes.2002

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesth Analg 95

      Pages: 1637-1644

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Two-person technique for fiberscope-aided tracheal intubation in a patient with a long and narrow retropharyngeal air space.2001

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesth Analg 92

      Pages: 1611-1613

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Propofol increases myofilament Ca^<2+> sensitivity and intracellular pH via activation of Na^+-H^+ exchange in rat ventricular myocytes.2001

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesthesiology 94

      Pages: 1096-1104

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The three-dimentional computed tomography imaging and prediction of unanticipated difficult tracheal intubation.2001

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesth Analg 92

      Pages: 281-282

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Atropine for the treatment of hiccup after laryngeal mask insertion.2001

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesth Analg 93

      Pages: 791-792

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The three-dimensional computed tomography imaging and prediction of unanticipated difficult tracheal intubation.2001

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesth Analg 92

      Pages: 281-282

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 麻酔科医とコンサルテーション-他科からの相談・依頼に対する適正な対応と実際-(並木昭義, 表 圭一 編)2002

    • Author(s)
      金谷憲明
    • Total Pages
      189
    • Publisher
      克誠堂出版株式会社、東京
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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