Functional analysis of macrophage in placental coagulation and intrauterine growth restriction

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 12671590
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Hamamatsu University, School of Medicine
• Principal Investigator: SUGIMURA Motoi associate Professor, 医学部附属病院, 助教授 (30273189)
• Co-Investigator(Kenkyū-buntansha): KANAYAMA Naohiro Professor, 医学部, 教授 (70204550) ; KOBAYASHI Takao associate Professor, 医学部, 助教授 (20107808)
• Project Period (FY): 2000 – 2002
• Keywords: macrophage / MIF / phosphatidylserin / antisense MIF adenovirus / protein C / intrauterine growth restriction model in mice

Research Abstract

In this research project, in order to establish the research tool for the investigation of function of macrophage in placenta, we established phosphatidylserin (PS)-induced intrauterine growth restriction (IUGR) mice model first. Then, we developed the recombinant adenovirus vector bearing sense and antisense macrophage migration inhibitory factor cDNA.
In the PS-induced IUGR model, exogenous anticoagulants, annexin V, activated protein C and heparin inhibited significantly the growth restriction in mice model, indicating that the model is associated with the hypercoagulable state by PS.Furthermore, in the BCG-lipopolysaccharide (LPS) induced liver failure model in mice, recombinant adenovirus vector bearing antisense macrophage migration inhibitory factor (MIF) cDNA suppressed significantly the damage in liver tissue suggesting that antisense MIF adenovirus vector modulates the BCG-LPS-induced inflammatory process through cytokine network in liver tissue.
We established the system for the analysis of macrophage functions by using recombinant virus vectors bearing sense and antisense MIF in animal model.