2002 Fiscal Year Final Research Report Summary
A MOLECULAR BIOLOGICAL STUDY ABOUT OLFACTORY DISTURBANXCE DUE TO OLFACTORY MUCOUS ELECTROLYTE DISORDER
| Project/Area Number |
12671655
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | KANAZAWA UNIVERSITY |
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Principal Investigator |
NISHIMURA Toshiro KANAZAWA UNIVERSITY HOSPITAL, DEPARTMENT OF OTOLARYNGOLOGY, ASSISTANT PROFESSOR, 医学部附属病院, 講師 (80251958)
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| Co-Investigator(Kenkyū-buntansha) |
石丸 正 金沢大学, 医学部附属病院, 助手 (10272965)
三輪 高喜 金沢大学, 大学院・医学系研究科, 助教授 (20229909)
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| Project Period (FY) |
2000 – 2002
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| Keywords | olfactory mucosa / olfactory mucous / Na^+ / K^+ ATPase |
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| Research Abstract |
Systemic or topical application of glucocorticoid is the treatment of choice for olfactory disturbance, but little is known about the mechanism of action. An animal model was produced by an intra-nasal application of 5% 2nSO_4 solution to Wistar rats. Dexamethasone was injected intra-peritoneally (0.01 mg/100g) for 14 days after the insult. Histologically, the regeneration process was completed on day 14 in both dexamethasone-injected and saline-injected control rats. Na^+/K^+ ATPase and glucocorticoid receptor immunoreactivity in olfactory mucosa of dexamethasone-injected rats was a little stronger than control rats. A quantitative polymerase chain reaction method was used to evaluate the expression. In dexamethasone-injected rats, up-regulation of glucocorticoid receptor mRNA (95% more than control rats, p=0.00068, unpaired t-test) and, of Na^+/K^+ ATPase mRNA expression (76% more than control rats, p=0.0042) was observed on day 14. The increased Na^+/K^+ ATPase expression along the basolateral surface of sustentacular cells was considered to be beneficial for an active uptake of K^+ around olfactory neurons that is released during excitation and the trans-epithelial absorption of Na^+ from olfactory mucous. Thus dexamethasone may contribute the improvement of olfactory disturbance at least, in part, through its receptor by means of regulation of the ionic concentration in the olfactory mucosal microenvironment.
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