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2001 Fiscal Year Final Research Report Summary

Molecular Mechanism of Barrier Function Changes of Epithelial Tight Junction in Allergic Rhinitis

Research Project

Project/Area Number 12671660
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionMie University

Principal Investigator

TAKEUCHI Kazuhiko  Mie Univ., Faculty of Medicine, Lecturer, 医学部・附属病院, 講師 (50206942)

Co-Investigator(Kenkyū-buntansha) KISHIOKA Chikako  Mie Univ., Faculty of Medicine, Assistant, 医学部, 助手 (10314113)
MAJIMA Yuichi  Mie Univ., Faculty of Medicine, Professor, 医学部, 教授 (60024791)
Project Period (FY) 2000 – 2001
Keywordsepithelium / permeability / tight junction / thrombin / leukotrienes / transepithelial resistance / histamine / allergic rhinitis
Research Abstract

1. We examined the effect of histamine, leukotriene D4 (LTD4) and thrombin on the epithelial permeability to 14C labeled mannitol and albumin using Calu-3 cell lines. 10^<-6>M LTD4 did not change permeability to mannitol or albumin. On the other hand, histamine and 10^<-7>〜10^<-8> M thrombin significantly increased epithelial permeability to mannitol. Moreover, 10^<-4>M histamine significantly increased the passage of horseradish peroxidase across epithelial tight junction in guinea pig nose.
2. Next we examined if histamine and thrombin can alter mRNA expression of tight junction proteins. We added histamine or vehicle to cultured human nasal epithelial cells and extracted RNA from them 4 hours later. After DNase treatment, mRNAs of β-actin and ZO-1 tight-junction protein were amplified by RT-PCR. Histamine significantly downregulated ZO-1 mRNA expression. This effect was blocked by pretreatment with mepyramine, an H1 receptor antagonist. Next we examined the effect of thrombin on the tight junction proteins mRNAs expression in cultured epithelial cells. Thrombin significantly decreased ZO-1 mRNA expression of the cells, but had no effect on the expression of occludin or claudin mRNAs. The reduction of ZO-1 mRNA by histamine and thrombin may cause increased permeability of the mucosa during allergic reactions in the nose.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] Kazuhiko Takeuchi, et al.: "Histamine Alters Gene Expression in Cultured Human Nasal Epithelial Cells"J Allergy Clin Immunol. 107. 310-314 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuhiko Takeuchi, et al.: "Histamine Alters Gene Expression in Cultured Human Nasal Epithelial Cells"J Allergy Clin Immunol. 107(2). 310-314 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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