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2002 Fiscal Year Final Research Report Summary

The Vestibular Ocular Reflex of the mutant mice deficient in delta 2 Glutamate receptor Subunit after unilateral labyrinthectomy

Research Project

Project/Area Number 12671662
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TSUJI Jun  Department of Otolaryngology - Head and Neck Surgery, Kyoto University Graduate School of Medicine, Assistant, 医学研究科, 助手 (30252448)

Co-Investigator(Kenkyū-buntansha) HIRANO Tomoo  Department of Biophysics, Graduate School of Science, Kyoto University, Professor, 理学研究科, 教授 (50181178)
NAITO Yasushi  Department of Otolaryngology - Head and Neck Surgery, Kyoto University Graduate School of Medicine, Lecturer, 医学研究科, 講師 (70217628)
Project Period (FY) 2000 – 2002
Keywordsdelta-2 subunit of glutamate receptor / vestibular compensation / unilateral labyrinthectomy / vestibulo ocular reflex / c-Fos / medial vestibular nucleus / inferior vestibular nicleus / prepositus hypoglossal nucleus
Research Abstract

Ionotrophic delta-2 subunit of glutamate receptor is specifically expressed only in cerebellar Purkinje cells in the nervous system, and mutant mice deficient in delta 2 proteins have been raised (Kashiwabuchi 1995).
These mice did nor exhibit long term depression (LTD), which is one form of synaptic plasticity (Hirano 1995). We examined the postural righting reflex and vestibulo ocular reflex (VOR) after unilateral labyrinthectomy (UL) in these mutant mice, to investigate the involvement of LTD in vestibular compensation. And we also examined the c-Fos expression after unilateral ladyrinthectomy in these mutant mice, to investigate the morphological change during the vestibular compensation.
After UL in the knock out mice deficient in delta 2 glutamate receptor subunit, the recovery of the postural righting reflex and VOR are retarded than normal mice, which suggests delta 2 glutamate receptor subunit plays an important role in the process of the vestibnlar compensation. After UL in the knock out mice deficient in delta 2 Glutamate Receptor Subunit, c-fos positive cells are greater in number in the contralateral sides in Mve and SpVe than normal, very few in the contralateral PrH and positive in ipsilesionai IO, contrary to normal mice. We assume that the in the knock-out mice which have dysfunctions in the floccules, the neurons in the vestibular nucleus on the contra latreal side became hyperactive because of the disinhibition from the cerebellum. And in knock out mice lacking control from the cerebellar floccules, c-fos was not activated in PrH on this side.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 村井紀彦, 内藤泰, 船曳和雄, 加藤尚美, 伊藤壽一: "振子様回転中の視・前庭相互作用によるvisual suppression検査"Equilibrium Research. 60巻4号. 234-240 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 村井紀彦, 辻 純, 堀龍介, 船曳和雄, 内藤泰, 中川隆之, 伊藤壽一: "マウスにおける一側内耳破壊後の前庭眼反射の代償"Equilibrium Research. 61巻1号. 40-44 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 辻 純, 村井紀彦, 内藤泰, 伊藤壽一: "一側内耳破壊後のマウス脳幹内c-Fos発現様式"Equilibrium Research. 61巻4号. 206-209 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Funabiki K, Naito Y: "Validity and limitation of detection of peripheral vestibular imbalance from analysis of manually rotated vestibulo-ocular reflex recorded in the routine vestibular clinic"Acta Otolaryngol. 122巻1号. 31-36 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakagawa T, Kim T, Murai N, Endo T, Iguchi F, Tateya I, Yamamoto N, Naito Y, Ito J: "A novel technique for inducing local inner ear damage"Hearing Research. 176巻1-2号. 122-127 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Norihiko Murai, Yasushi Naito, Kazuo Funabiki, Naomi Kato, Juichi Ito: "Visual suppression testing during manual rotation"Equilibrium Researeh. 60. 234-240 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Norihiko Murai, Jun Tsuji, Ryusuke Hori, Kazuo Funabiki, Takayuki Nakagawa, Juichi Ito: "Vestibular cimpensation after unilateral labyrinthectomy in mice"Equilibrium Research. 61. 40-44 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jun Tsuji, Norihiko Murai, Yasushi Naito, Juichi Ito: "c-Fos expression in the mouse brainstem after unilateral labyrinthectomy"Equilibrium Research. 61. 206-209 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Funabiki K, Naito Y: "Validity and limitation of detection of peripheral vestibular imbalance from analysis of manually rotated vestibulo-ocular reflex recorded in the routine vestibular clinic"Acta Otolaryngol. 122. 31-36 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakagawa T, Kim T, Murai N, Endo T, Iguchi F, Tateya I, Yamamoto N, Naito Y, Ito J: "A novel technique for inducing local inner ear damage"Hearing Research. 176. 122-127 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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