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2001 Fiscal Year Final Research Report Summary

Studies on the carcinogenesis of rhinosinus in relation to reactive oxygen species

Research Project

Project/Area Number 12671664
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKagoshima University

Principal Investigator

TAKEUCHI Toru  Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (00188161)

Co-Investigator(Kenkyū-buntansha) XU Baohui  Kagoshima University, Faculty of Medicine, Research Associate, 医学部, 助手 (00264408)
AOYAMA Koji  Kagoshima University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (70117472)
MIYAGUCHI Mamoru  Higashiosaka city general hospital, Otorhinolaryngology, Head, 耳鼻咽頭科, 部長 (70166130)
Project Period (FY) 2000 – 2001
KeywordsReactive Oxygen Species / Oxidative DNA damage / Cancer in the rhinosinus / Chronic inflammation / Carcinogenesis / Human
Research Abstract

We have established a method to determine a typical oxidative DNA damage, 8-hydroxydeoxyguanosine (8OHdG) in the rhinosinus mucosae. That is, extraction of DNA from the mucosae, digestion of DNA to nucleosides with nuclease P1 and alkaline phosphatase, ultra filtration of the nucleosides, HPLC separation of the nucleoside with reversed-phase column, and the detection of 8OHdG with electrochemical detector (ECD). We did the steps from DNA extraction to nucleosides ultra filtration in an anaerobic incubator to reduce the artificial formation of 8OHdG during sample processing.
With the method, we determined the levels of 8OHdG in the rhinosinus mucosae from the patients with chronic rhinosinusitis, nasal polyps and papilloma of rhinosinus, and compared the 8OHdG levels with those in normal rhinosinus mucosae. The diagnosis was done microscopically. The samples were obtained from the patients who had been scheduled to remove lesions by operation in Higashiosaka city hospital and had given us the informed consent.
The 8OHdG levels were 0.54 in normal mucosae(n=4), 0.59 in nasal polyp(n=7), 0.48 in chronic rhinosinusitis (n=7), and 0.43 in papilloma (n=3). We could not find significant difference between normal and other groups.
This is the first study to determine 8OHdG in rhinosinus mucosae. Ultrafiltration was effective to remove contaminants which interfere the 8OHdG analyses by HPLC-ECD, However we could not find significant increase of 8OHdG in chronic rhinosinusitis related samples. Recently the incidence of cancer in the rhinosinus is decreasing. From the present study we considered that the grades of chronic inflammation in rhinosinusitis are not severe enough to induce 8OHdG due to the improvement of the treatments and/or the host status.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Lu, Y., et al.: "Genotoxic effects of a-endosulfan and a-endosulfan on human Hep G2 cells"Environ. Health Perspect.. 108. 559-561 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeuchi, T., et al.: "Mechanism of oxidative DNA damage inducation in a strict anaerobe, Prevotella melaninogenica"FEMS Microbiol. Left.. 192. 133-138 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiozaki, H., et al.: "Larynx preservation in surgical treatment of cervical esophageal cancer -combined procedure of laryngeal suspension and cricopharyngeal myotomy"Diseases of the Esophagus. 13. 213-218 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanno, H., et al.: "Low frequency of HLA-A^*0201 allele in patients with Epstein-Barr virus-positive nasal lymphomas with polymorphic reticulosis morphology"Int-J-Cancer.. 87. 195-199 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mikulowska-Mennis, A., et al.: "Lymphocyte Migration to inflamed Lacrimal Glands Is Mediated by Vascular Cell Adhesion Molecule-1/a4 a1 Integrin, Peripheral Node Addressin/L-Selectin, and Lymphocyte Function-Associated Antigen-1 Adhesion Pathways"American Journal of Pathology. 159. 671-681 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹内亨: "活性酸素と健康-活性酸素ってなんだろう-がんとの関連から"鹿児島市医報. 40. 8-26 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹内亨: "別冊医学のあゆみ,酸素高感受性生体システム(嫌気性菌)における酸化ストレスin酸化ストレス-フリーラジカル医学生物学の最前線"医歯薬出版. 4(326) (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lu,Y., Takeshita,T., Takeuchi,T. and Morimoto,K.: "Genotoxic effects of a-endosulfan and a-endosulfan on human Hep G2 cells"Environ. Health Perspect. 108. 559-561 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takelchi,T., Kato,N., Watanabe,K. and Morimoto,K.: "Mechanism of oxidative DNA damage inducation in a strict anaerobe, Prevotella melaninogenica"FEMS Microbiol. Lett. 192. 133-138 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiozaki,H., Tsujinaka,T., Inoue,M,, Yano,M., Doki,Y., Miyaguchi.M., Inoue,T., Hosokawa,K. and Monden,M: "Larynx preservation in surgical treatment of cervical esopnageai cancer - combined procedure of laryngeal suspension and cricopharyngeal myotomy"Diseases of the Esophagus. 13. 213-218 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanno,H., Kojya,S., Li,T., Ohsawa,M., Nakatsuka,S.,Miyaguchi,M., Harabuchi,Y., Aozasa,K.: "Low frequency of HLA-A*0201 allele in patients with Epstein-Barr virus-positive nasal lymphomas with polymorphic reticulosis morphology"Int-J-Cancer. 87. 195-9 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Anna,Mikulowska-Mennis, Baohui,Xu, John,M.Berberian, and Sara,A.Michie.: "Lymphocyte Migration to Inflamed Lacrimali Glands Is Mediated by Vascular Cell Adhesion Molecule-1/a4 a1 Integrin, Peripheral Node Addressin/L-Selectin, and Lymphocyte Function-Assotiated Antigen-1 Adhesion Pathways"American Journal of Pathology. 159. 671-681 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeuchi,T.: "Health Effects ot Reactive Oxygen species-In Relation to Carcinogenesis"J. Kagoshima City Medical Association. 40. 8-26 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeuchi,T.: "Oxidative stress in oxygen sensitive organisms, anaerobes"In Oxidative stress-Recent advance in free radical biology and medicine Igaku no Ayumi, Ishiyaku Publishers. 48-51 (2001)

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      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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